Description
Ethidium bromide is a phenanthridine dye of minimum 98 % purity used as a nucleic-acid stain in molecular biology. It is identified by CAS number 1239-45-8 and empirical formula C₂₁H₂₀BrN₃, with a molar mass of 394,33 g/mol, a density of 1,01 g/cm³ and a flash point above 100 °C. The compound intercalates double-stranded DNA and RNA and is employed for visualisation of nucleic acids after gel electrophoresis under appropriate detection conditions. Laboratories use ethidium bromide where a classical intercalating dye of defined purity is specified for DNA and RNA detection workflows. The product is listed under EG number 214-984-6 and belongs to standard molecular-biology reagent sets that require carefully handled fluorescent nucleic-acid stains of documented assay.
This article is part of Fluorescent dyes & labels, in Dyes, stains & indicators.
Key features
- Appearance: red, cryst. powder
- Assay: ≥98.0 %
- Solubility (in H2O): clear, red
- pH value (2 % in H2O): 4.0-7.0
- Loss on drying: ≤10.0 %
- CAS number: 1239-45-8
- Pack sizes: 1 g, 5 g
- EG nr.: 214-984-6
- Purity: min. 98 %
- Molar mass: 394,33 g/mol
- Technical Information Sheet:
- Safety Data Sheet:
Compliance & Safety Information
DangerHazard Statements (H-Phrases)
Precautionary Statements (P-Phrases)
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Questions about Ethidium bromide, min. 98 %, 1 g, glass
What is this article?
Ethidium bromide, min. 98 %, 1 g, glass is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Fluorescent dyes & labels, inside Dyes, stains & indicators, in Lab chemicals.
A fluorescent dye absorbs at one wavelength and emits at a longer one. As a label it is attached to a protein, a nucleic acid or a cell. Excitation and emission maxima, and the reactive group if it must conjugate, are the specification. The colour name does not tell you the wavelengths.
A dye or a stain binds, or changes colour, so that something invisible becomes visible. An indicator changes at a pH or a redox point. A fluorescent dye absorbs one wavelength and emits another, which is how a microscope or a plate reader picks it out. The excitation and emission, or the transition interval, are the specification. The colour name on the label is not. Structures are on PubChem under IUPAC names. Methods are in the PubMed literature.
Which category should I open next?
The category is Dyes, stains & indicators. The sub-category is Fluorescent dyes & labels. The catalogue is Lab chemicals.
Shelves beside this one are Microscopy stains & staining solutions and Dyes & pigments.
What should I check before I order?
The technical information on this page is the manufacturer's sheet. Read that, not a rewritten specification.
When this page shows a safety data sheet, use that manufacturer file. Hazard text does not belong in the sales description. For a pack size, a grade or a certificate that is not on the page, send a quote request or contact the team.
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The seven catalogues are Labware, Lab chemicals, Lab instruments, Lab consumables, Chromatography equipment, Lab safety equipment and Life Science & Microbiology.
