Description
ROTI®Garose-His/Ni HPBeads is a chromatography medium supplied for biochemistry applications, in particular affinity-based separation workflows involving His-tagged targets on a nickel-charged matrix. The high-performance bead format is intended for bio-chromatography setups where selective binding and elution steps are required in protein purification or related laboratory protocols. Physical characteristics include a density in the range 0,85 to 0,95 g/cm³, a flash point above 50 °C and a boiling point above 80 °C, supporting controlled handling under standard laboratory conditions. The preparation is Offered by manufacturer Carl ROTH within the ROTI®Garose line, these His/Ni HPBeads integrate into established biochemistry and affinity chromatography procedures used for recombinant protein isolation and related analytical tasks. Unit weight is 0,080 kg. Prepare, equilibrate and operate the medium according to the laboratory’s chromatography protocol and good practice for nickel-affinity resins so that binding capacity and elution behaviour remain consistent with the intended biochemical application.
This article is part of Sorbents, silica gel & ion exchangers, in SPE & sample preparation.
Key features
- Particle size: 50-150 µm
- Beads (cross-linked agarose): 6 %
- Ligand: IDA
- Metal ion: nickel
- Loading capacity (gel) (Ni2+): ~20 µmol/ml
- Size exclusion limit (Da): ~4x106
- Ethanol: 20 %
- Pack sizes: 25 ml, 100 ml
- Density: 0,85 to 0,95 g/cm³
- Flash point: >50 °C
- Storage temperature: +4 °C
- Product type: ROTI®Garose-His/Ni HPBeads
- Technical Information Sheet:
- Safety Data Sheet:
Compliance & Safety Information
WarningHazard Statements (H-Phrases)
Precautionary Statements (P-Phrases)
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Questions about ROTI®Garose-His/Ni HPBeads, for biochemistry, 25 ml, plastic
What is this article?
ROTI®Garose-His/Ni HPBeads, for biochemistry, 25 ml, plastic is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Sorbents, silica gel & ion exchangers, inside SPE & sample preparation, in Chromatography equipment.
Loose sorbent, silica gel and ion-exchange resin are the phases bought in bulk to pack a column or to stir into a solution. Particle size and activity, or the ion-exchange capacity, are the specification. A TLC silica and a flash silica of different particle sizes are not the same separation.
Solid-phase extraction holds a compound on a sorbent while the rest of the sample is washed away, then releases it in a small volume. The sorbent, the bed mass and the solvents are the method. Silica, ion exchangers and polymer phases do different jobs. A flash column is the same idea scaled toward purification rather than trace cleanup. The molecules are on PubChem, named by IUPAC.
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The category is SPE & sample preparation. The sub-category is Sorbents, silica gel & ion exchangers. The catalogue is Chromatography equipment.
Shelves beside this one are SPE cartridges & columns and Flash, empty & glass chromatography columns.
What should I check before I order?
The technical information on this page is the manufacturer's sheet. Read that, not a rewritten specification.
When this page shows a safety data sheet, use that manufacturer file. Hazard text does not belong in the sales description. For a pack size, a grade or a certificate that is not on the page, send a quote request or contact the team.
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