Description
ROTI®Mark TRICOLOR XTRA, Protein marker, prestained, ready-to-use, Density (D) ~1 g/cm³, Storage temp. -20 °C
Perfect separation behaviour in the gel. Protein ladders from Carl ROTH are acylated and pre-reduced, for optimal running behaviour in the gel. For each batch, intensity of bands is optimised and electrophoretically tested. All protein ladders are offered as ready-to-use solutions which may be applied directly to the gel.
Please note: Reliable size determination of proteins cannot be performed using prestained markers. For this, we recommend the use of an unstained marker.
ROTI®Mark TRICOLOR XTRA is a tricolour-prestained ladder covering an extra range of molecular weights from 10 to 310 kDa in Tris-Glycine buffer. In order to visualise separation during the gel run and for better orientation in the gel, there is, besides the blue coloured proteins, one protein prestained in green (25 kDa) and two proteins in red (75 and 310 kDa). All bands excel by having a very even running behaviour and a good differentiation.ROTI®Mark TRICOLOR XTRA is an ideal size ladder for controlling the gel run during SDS-PAGE and for reliable evaluation of transfer efficiency during Western Blot.
Fig.: 15 % SDS-PAGE (Tris-Glycine)
| Number of protein bands | 12 |
| Amount of traces | ~200/ml |
| Colour | blue prestained |
| Size range | 10-310 kDa |
| Detection in western blot | no detection |
| Quantification | non recommendé |
| Reference bands | 25 kD green, 75 and 310 kD red |
| Dispatch | ready-to-use |
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Questions about ROTI®Mark TRICOLOR XTRA, Protein marker, prestained, 250 µl, plastic
What is this article?
ROTI®Mark TRICOLOR XTRA, Protein marker, prestained, 250 µl, plastic is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Protein extraction, purification & markers, inside Protein biology & Western blot, in Life Science & Microbiology.
Extraction reagents break the cell and keep the protein soluble. Purification resins and columns, often an affinity tag such as His, pull one protein out. A marker is a set of known masses run in the next lane. The mass range of the marker has to cover the protein being sought.
Protein work separates a mixture, then asks which protein is which. Extraction and purification take the protein out of the cell. A marker shows the masses. A Western blot moves the separated proteins onto a membrane and probes them with an antibody. The membrane, nitrocellulose or PVDF, has to bind the protein and survive the washes. Antibodies and methods are in PubMed. Reference material is at ATCC.
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The category is Protein biology & Western blot. The sub-category is Protein extraction, purification & markers. The catalogue is Life Science & Microbiology.
Shelves beside this one are Blotting membranes & papers and Protein biology (other).
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