Description
ROTI®Nylon 0.45, transfer membrane, neutral charged, sheets, 20 x 20 cm, pore size 0.45 µm
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The optimal membrane for DNA/RNA transfer, especially colony and plaque lifting and all multiple rehybridisations.ROTI®Nylon 0.45 is a neutral, reinforced nylon membrane. Like all ROTI®Nylon membranes, it has a very high tensile strength and is highly resistant to alcohols, acetone and many other organic solvents.ROTI®Nylon 0.45 is not charged at buffer pH 6.5 and has excellent hydrophilic properties due to the amino and carboxy groups on the surface. The membrane charge (ζ-potential) can be adjusted by the pH value of the buffer system (positive membrane charge at pH<6.5; negative membrane charge at pH>6.5). Steam sterilisation is possible.The covalent binding of transferred nucleic acid is achieved by crosslinking (UV crosslinking for 20 s at 260 nm). The excellent signal/background ratio of the membrane then enables highly sensitive detections (single copy gene detection) and multiple rehybridisations without loss of signal quality.
- Southern and Northern blotting
- Western blotting
- Microarrays and Macroarrays
- Dot/Slot blotting
| Dimensions | 20 × 20 cm |
| Pore size | 0,45 µm |
| Bond | hydrophile |
| Load | neutral/amphoteric |
| Thickness | 140-190 µm |
| Immobilisation | UV |
| Binding capacity | 350 µg/cm² |
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Questions about ROTI®Nylon 0.45, transfer membrane, neutral charged, sheets, 20 x 20 cm
What is this article?
ROTI®Nylon 0.45, transfer membrane, neutral charged, sheets, 20 x 20 cm is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Blotting membranes & papers, inside Protein biology & Western blot, in Life Science & Microbiology.
A blotting membrane, nitrocellulose or PVDF, binds proteins transferred out of a gel. Pore size, 0.2 µm or 0.45 µm, and the format, roll or pre-cut, are the choices. PVDF is activated in methanol. The papers and the sponges around it in the cassette are part of the same stack.
Protein work separates a mixture, then asks which protein is which. Extraction and purification take the protein out of the cell. A marker shows the masses. A Western blot moves the separated proteins onto a membrane and probes them with an antibody. The membrane, nitrocellulose or PVDF, has to bind the protein and survive the washes. Antibodies and methods are in PubMed. Reference material is at ATCC.
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The category is Protein biology & Western blot. The sub-category is Blotting membranes & papers. The catalogue is Life Science & Microbiology.
Shelves beside this one are Protein biology (other) and Protein extraction, purification & markers.
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