Description
ROTI®Nylon 0.45 transfer membrane is a neutrally charged nylon membrane supplied on a roll for blotting and nucleic acid transfer applications. It features a pore size of 0,45 µm and roll dimensions L 300 × W 30 cm, so users can cut membrane pieces to match gels, cassettes and transfer setups. Neutral charge supports transfer of nucleic acids to the membrane in Southern and Northern blot type procedures and related laboratory methods. This roll presentation from manufacturer Carl ROTH suits laboratories that need continuous 0,45 µm nylon membrane stock rather than fixed sheet sizes. The combination of 300 cm length and 30 cm width offers flexible sizing while retaining the ROTI®Nylon 0.45 grade and 0,45 µm pore specification. Pack size is one roll, with a stated weight of 0,290 kg. Intended for transfer of nucleic acids to membranes within blotting workflows, ROTI®Nylon 0.45 in roll form provides a practical bulk option for routine molecular biology work where a larger pore size grade and cut-to-size handling are preferred.
This article is part of Blotting membranes & papers, in Protein biology & Western blot.
Key features
- Pack size: 1 roll
- Product type: Nylon transfer membrane, roll
- Brand grade: ROTI®Nylon 0.45
- Pore size: 0,45 µm
- Charge: Neutral charged
- Dimensions: L 300 × W 30 cm
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Questions about ROTI®Nylon 0.45, transfer membrane, roll, neutral charged, roll, L 300 x W 30 cm
What is this article?
ROTI®Nylon 0.45, transfer membrane, roll, neutral charged, roll, L 300 x W 30 cm is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Blotting membranes & papers, inside Protein biology & Western blot, in Life Science & Microbiology.
A blotting membrane, nitrocellulose or PVDF, binds proteins transferred out of a gel. Pore size, 0.2 µm or 0.45 µm, and the format, roll or pre-cut, are the choices. PVDF is activated in methanol. The papers and the sponges around it in the cassette are part of the same stack.
Protein work separates a mixture, then asks which protein is which. Extraction and purification take the protein out of the cell. A marker shows the masses. A Western blot moves the separated proteins onto a membrane and probes them with an antibody. The membrane, nitrocellulose or PVDF, has to bind the protein and survive the washes. Antibodies and methods are in PubMed. Reference material is at ATCC.
Which category should I open next?
The category is Protein biology & Western blot. The sub-category is Blotting membranes & papers. The catalogue is Life Science & Microbiology.
Shelves beside this one are Protein biology (other) and Protein extraction, purification & markers.
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