Description
Roti®Nylon Plus Reprobe is a positively charged nylon membrane for transfer of nucleic acids in blotting workflows. With a pore size of 0,45 µm, it is intended for Southern and Northern blot procedures in which DNA or RNA is moved from gels onto a membrane support for further analysis. The roll measures 300 cm in length and 30 cm in width, so sheets can be cut to match common gel formats and transfer assemblies. Pack size is one roll, giving flexible material for routine molecular biology work. Roti®Nylon Plus Reprobe from manufacturer Carl ROTH belongs to the blotting range used for nucleic acid transfer to membranes. The positively charged nylon surface and defined 0,45 µm pore size support standard handling in transfer protocols. Laboratories can prepare custom pieces from the continuous roll, which helps adapt membrane size to different experimental layouts without relying on fixed sheet formats alone. This configuration suits day-to-day Southern and Northern blotting setups that require a positively charged nylon membrane in roll form.
This article is part of Blotting membranes & papers, in Protein biology & Western blot.
Key features
- Pack size: 1 roll
- Membrane type: Positively charged nylon
- Pore size: 0,45 µm
- Dimensions: 300 cm × 30 cm
- Format: Roll
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Questions about Roti®Nylon Plus Reprobe, pos. charged, L300 x W30 cm, 0.45 µm, 1 roll(s)
What is this article?
Roti®Nylon Plus Reprobe, pos. charged, L300 x W30 cm, 0.45 µm, 1 roll(s) is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Blotting membranes & papers, inside Protein biology & Western blot, in Life Science & Microbiology.
A blotting membrane, nitrocellulose or PVDF, binds proteins transferred out of a gel. Pore size, 0.2 µm or 0.45 µm, and the format, roll or pre-cut, are the choices. PVDF is activated in methanol. The papers and the sponges around it in the cassette are part of the same stack.
Protein work separates a mixture, then asks which protein is which. Extraction and purification take the protein out of the cell. A marker shows the masses. A Western blot moves the separated proteins onto a membrane and probes them with an antibody. The membrane, nitrocellulose or PVDF, has to bind the protein and survive the washes. Antibodies and methods are in PubMed. Reference material is at ATCC.
Which category should I open next?
The category is Protein biology & Western blot. The sub-category is Blotting membranes & papers. The catalogue is Life Science & Microbiology.
Shelves beside this one are Protein biology (other) and Protein extraction, purification & markers.
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