Description
ROTI®ProBlot NC Blotting Sandwich is a pre-assembled nitrocellulose blotting sandwich for transfer of nucleic acids in Southern and Northern blot workflows. Each unit measures 70 × 84 mm and uses a pore size of 0,2 µm, matching compact gel formats common in molecular biology. The pack contains 20 pc(s) with a total weight of 0,704 kg, giving laboratories a convenient multi-piece supply for repeated transfer runs. This ROTI®ProBlot sandwich from manufacturer Carl ROTH is intended for membrane-based nucleic acid immobilisation where nitrocellulose binding behaviour is preferred. Pre-cut sandwich format simplifies set-up in the transfer stack and supports consistent alignment with mini-gel dimensions. The 0,2 µm pore size is suited to protocols that specify finer nitrocellulose membranes for nucleic acid retention. Suitable for routine Southern and Northern blotting and related transfer-of-nucleic-acids applications that call for 70 × 84 mm NC sandwiches at 0,2 µm.
This article belongs on Blotting membranes & papers, in Protein biology & Western blot.
Key features
- Pack size: 20 pc(s)
- Product type: NC blotting sandwich
- Material: Nitrocellulose
- Pore size: 0,2 µm
- Dimensions: 70 × 84 mm
- Technical Information Sheet:
- Safety Data Sheet:
Compliance & Safety Information
Danger
Hazard Statements (H-Phrases)
Precautionary Statements (P-Phrases)
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Questions about ROTI®ProBlot NC Blotting Sandwich, Nitrocellulose, 70 x 84 mm, Pore size 0.2 µm
What is this article?
ROTI®ProBlot NC Blotting Sandwich, Nitrocellulose, 70 x 84 mm, Pore size 0.2 µm is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Blotting membranes & papers, inside Protein biology & Western blot, in Life Science & Microbiology.
A blotting membrane, nitrocellulose or PVDF, binds proteins transferred out of a gel. Pore size, 0.2 µm or 0.45 µm, and the format, roll or pre-cut, are the choices. PVDF is activated in methanol. The papers and the sponges around it in the cassette are part of the same stack.
Protein work separates a mixture, then asks which protein is which. Extraction and purification take the protein out of the cell. A marker shows the masses. A Western blot moves the separated proteins onto a membrane and probes them with an antibody. The membrane, nitrocellulose or PVDF, has to bind the protein and survive the washes. Antibodies and methods are in PubMed. Reference material is at ATCC.
Which category should I open next?
The category is Protein biology & Western blot. The sub-category is Blotting membranes & papers. The catalogue is Life Science & Microbiology.
Shelves beside this one are Protein biology (other) and Protein extraction, purification & markers.
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