Description
ROTI®ProBlot PVDF Blotting Sandwich is a polyvinylidene fluoride blotting sandwich intended for transfer of nucleic acids onto membranes in Southern and Northern blot procedures. The membrane is specified with a pore size of 0,2 µm and measures 70 x 84 mm, matching compact gel and transfer formats. Supplied as 20 pc(s) per pack, it facilitates repeatable stack assembly for molecular biology laboratories running nucleic-acid blotting. PVDF is a established support for DNA and RNA immobilisation where mechanical strength and consistent transfer behaviour are required. This configuration from manufacturer Carl ROTH is presented as a ready blotting sandwich to simplify setup compared with cutting loose membrane sheets. Approximate pack weight is 0,080 kg. Use in Southern and Northern protocols for transfer of nucleic acids to membranes when the stated 70 x 84 mm size and 0,2 µm pore size correspond to the laboratory’s gel and blotting equipment.
This article belongs on Blotting membranes & papers, in Protein biology & Western blot.
Key features
- Pack size: 20 pc(s)
- Membrane material: Polyvinylidene fluoride (PVDF)
- Pore size: 0,2 µm
- Dimensions: 70 x 84 mm
- Format: Blotting sandwich
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Questions about Blotting-Sandwich ROTI® ProBlot PVDF 0.45
What is this article?
Blotting-Sandwich ROTI® ProBlot PVDF 0.45 is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Blotting membranes & papers, inside Protein biology & Western blot, in Life Science & Microbiology.
A blotting membrane, nitrocellulose or PVDF, binds proteins transferred out of a gel. Pore size, 0.2 µm or 0.45 µm, and the format, roll or pre-cut, are the choices. PVDF is activated in methanol. The papers and the sponges around it in the cassette are part of the same stack.
Protein work separates a mixture, then asks which protein is which. Extraction and purification take the protein out of the cell. A marker shows the masses. A Western blot moves the separated proteins onto a membrane and probes them with an antibody. The membrane, nitrocellulose or PVDF, has to bind the protein and survive the washes. Antibodies and methods are in PubMed. Reference material is at ATCC.
Which category should I open next?
The category is Protein biology & Western blot. The sub-category is Blotting membranes & papers. The catalogue is Life Science & Microbiology.
Shelves beside this one are Protein biology (other) and Protein extraction, purification & markers.
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