Description
ROTI®ProBlot PVDF Blotting Sandwich is a ready-to-use blotting sandwich based on polyvinylidene fluoride membrane with a pore size of 0,45 µm and sheet dimensions of 85 × 135 mm. It is intended for transfer of nucleic acids to membranes in Southern and Northern blot workflows. The pre-assembled sandwich format simplifies membrane handling and supports consistent set-up of transfer stacks in molecular biology laboratories. PVDF provides mechanical strength suited to immobilisation and subsequent hybridisation steps. Packs contain 20 pieces and weigh about 0,155 kg, matching common mini-gel blot sizes used in routine nucleic acid analysis. Available from manufacturer Carl ROTH, the sandwich integrates into electrophoretic or capillary transfer protocols for applications such as restriction mapping, gene-expression studies, and hybridisation-based detection. The 0,45 µm pore size supports retention across a practical range of fragment lengths while allowing buffer exchange during transfer. Using a defined sandwich reduces cutting and alignment work compared with assembling loose membrane and filter layers for every blot, helping laboratories maintain reproducible blotting practice.
This article is part of Blotting membranes & papers, in Protein biology & Western blot.
Key features
- Pack size: 20 pc(s)
- Material: Polyvinylidene fluoride (PVDF)
- Pore size: 0,45 µm
- Dimensions: 85 × 135 mm
- Format: Blotting sandwich
- Weight: 0,155 kg
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Questions about Blotting-Sandwich ROTI® ProBlot PVDF 0.45
What is this article?
Blotting-Sandwich ROTI® ProBlot PVDF 0.45 is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Blotting membranes & papers, inside Protein biology & Western blot, in Life Science & Microbiology.
A blotting membrane, nitrocellulose or PVDF, binds proteins transferred out of a gel. Pore size, 0.2 µm or 0.45 µm, and the format, roll or pre-cut, are the choices. PVDF is activated in methanol. The papers and the sponges around it in the cassette are part of the same stack.
Protein work separates a mixture, then asks which protein is which. Extraction and purification take the protein out of the cell. A marker shows the masses. A Western blot moves the separated proteins onto a membrane and probes them with an antibody. The membrane, nitrocellulose or PVDF, has to bind the protein and survive the washes. Antibodies and methods are in PubMed. Reference material is at ATCC.
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The category is Protein biology & Western blot. The sub-category is Blotting membranes & papers. The catalogue is Life Science & Microbiology.
Shelves beside this one are Protein biology (other) and Protein extraction, purification & markers.
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