Description
Roti-Spin MINI 100 kDa, 25 pcs, Centrifugal devices
For purification and concentration of biomolecules and for purification of solutions.
- ROTI®Spin MINI: 1.5 ml sample tube for up to 500 µl sample
- ROTI®Spin MIDI: 15 ml sample tube for up to 5 ml sample
- ROTI®Spin MAXI: 50 ml sample tube for up to 15 ml sample
Centrifugal filters for ultrafiltration of biomolecules. The membranes made of highly chemical-resistant polyethersulfone have been tightly sealed to prevent leakage and loss of samples. Furthermore, the membranes underwent special treatment minimising the binding of biomolecules, in order to optimise sample recovery rates.ROTI®Spin Centrifugal filters can be applied to a variety of applications and can be used to replace tedious and labour-intensive methods like gel filtration, gel purification or precipitation. Application is time-saving and very simple - only few centrifugal steps are necessary to concentrate and recover your sample. During this, the sample remains untouched in the upper reservoir, and cross-contamination is efficiently prevented even when large numbers of samples are handled.
Desalting or buffer exchange following restriction digest, PCR or such; separation of free nucleotides and polymerised DNA; removal of contaminating compounds (like DNA) from stock solutions (e.g. PCR-buffers); concentration of DNA or proteins; separation of proteins of different size; sample preparation prior to HPLC.
| Overview for selecting the appropriate pore size | ||
| MWCO | Molecule size | DNA size |
| 10 kDa | 30 – 90 kDa | 50 – 200 bp |
| 30 kDa | 90 – 180 kDa | 200 – 500 bp |
| 50 kDa | 150 – 300 kDa | 500 bp – 1 kb |
| 100 kDa | >300 kDa | >1 kb |
Sample volumes:Initial volume: 50-500 μlFinal volume: 10-20 μlMWCO:Centrifugation units with membranes in 5 different molecular weight cut-offs (MWCO) are available for specific applications. Select the appropriate MWCO according to the following rule of thumb:
- Suitable for nucleic acids, proteins/peptides, other biomolecules >200 kDa, and very small bioparticles
- Replaces standard applications such as precipitation, dialysis, gel filtration, gel purification, column chromatography, gradient centrifugation, etc.
- Gentle: no shearing of DNA up to 100 kb, proteins remain native and enzymatically active
- Can be used without restriction on labeled samples (isotope, fluorescence, chromogenic labeling)
- Very high recovery rates: with a suitable MWCO, the yield is >90%.
| MWCO | 100 kDa |
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Questions about Roti-Spin MINI 100 kDa, 25 pcs, Centrifugal devices, 25 unit(s)
What is this article?
Roti-Spin MINI 100 kDa, 25 pcs, Centrifugal devices, 25 unit(s) is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Filtration accessories, inside Filtration, in Lab consumables.
Filtration accessories are the holders, the gaskets, the manifolds and the flasks a membrane or a Büchner setup needs. A holder for 47 mm discs does not clamp a 25 mm disc. The gasket is what actually seals.
Filtration holds back what is larger than the pores and lets the rest through. Paper is the ordinary laboratory filter. A membrane has a stated pore size and a polymer that must survive the solvent. A syringe filter is that membrane in a housing on a syringe. Glass fibre takes a higher dirt load. Ultrafiltration holds back macromolecules and passes salts. The pore size, the chemical compatibility and the volume are the three choices. Laboratory apparatus around filtration is indexed under ISO laboratory apparatus.
Which category should I open next?
The category is Filtration. The sub-category is Filtration accessories. The catalogue is Lab consumables.
Shelves beside this one are Filter paper, Sintered glass filter plates & discs, Syringe filters, Filter cartridges & capsules, Membrane filters, Glass & quartz fibre filters, Bottle-top & vacuum filtration units, Ultrafiltration & concentrators, Extraction thimbles and Dialysis tubing & devices.
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The technical information on this page is the manufacturer's sheet. Read that, not a rewritten specification.
When this page shows a safety data sheet, use that manufacturer file. Hazard text does not belong in the sales description. For a pack size, a grade or a certificate that is not on the page, send a quote request or contact the team.
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The seven catalogues are Labware, Lab chemicals, Lab instruments, Lab consumables, Chromatography equipment, Lab safety equipment and Life Science & Microbiology.