Description
Tris-Borate-EDTA buffer pH 8,3 (10×) GEN is a concentrated electrophoresis buffer concentrate supplied in a 1 L pack for molecular biology workflows. The 10× formulation is intended for dilution to working strength before use in nucleic acid separation and related gel electrophoresis procedures that require a Tris-borate-EDTA system buffered at pH 8,3. Net weight is 1,610 kg per pack, consistent with a one-litre liquid reagent presentation suitable for routine laboratory stock. This GEN-grade buffer from manufacturer Labbox supports standard agarose and polyacrylamide gel electrophoresis setups where TBE chemistry is specified, helping maintain ionic conditions during DNA and RNA fragment analysis. The ready concentrate format simplifies preparation of running buffer and gel buffer batches, reducing weighing and dissolution steps at the bench. Laboratories can aliquot and dilute the 10× stock according to their protocols to obtain the desired working concentration for horizontal or vertical electrophoresis systems. Packaged as a single 1 L unit, it is positioned for molecular biology reagent inventories that rely on defined TBE buffer at pH 8,3.
This article is part of Biological buffers (Tris, HEPES, MOPS), in Buffers & pH solutions.
Key features
- Pack size: 1 L
- Product type: Tris-Borate-EDTA buffer pH 8,3 (10×) GEN
- Concentration: 10× concentrate
- pH: 8,3
- EAN: 8434868060043
- Technical Information Sheet:
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Questions about TRIS-Borate-EDTA-Buffer pH 8.3 (10x) GEN, 1 L
What is this article?
TRIS-Borate-EDTA-Buffer pH 8.3 (10x) GEN, 1 L is the article on this page. Luminix Health distributes Labbox for laboratory use. It sits in Biological buffers (Tris, HEPES, MOPS), inside Buffers & pH solutions, in Lab chemicals.
Tris, HEPES, MOPS and their relatives hold pH in a range that cells and proteins tolerate, with a pKa near the working pH. The useful range is roughly one unit either side of the pKa. A phosphate buffer and Tris are not interchangeable in a method that names one of them, because the ion itself has effects.
A buffer resists a change in pH when a little acid or base is added. It does that because a weak acid and its conjugate base are both present. A pH buffer used to calibrate a meter has a stated value at a stated temperature. A biological buffer such as Tris or HEPES is chosen because it holds the pH of a living system and does not poison it. Conductivity standards are the sister articles for a conductivity meter. The ions are on PubChem. Electrode calibration practice sits with EURAMET and BELAC.
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The category is Buffers & pH solutions. The sub-category is Biological buffers (Tris, HEPES, MOPS). The catalogue is Lab chemicals.
Shelves beside this one are pH buffers & conductivity standards and Buffers (other).
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