ELISA kits

Start with the target and the species: a human kit does not reliably detect the mouse protein. Then check the sample types the kit is validated for, such as serum, plasma or cell culture supernatant, and whether its detection range covers the levels you expect. Those details are in each kit's datasheet, so ask for it before you order. Examples: the Human NGF ELISA kit, 48 assays, and the Mouse Hepcidin ELISA kit. Send your target, species and sample type to sales@luminixhealth.com and we will check the datasheet.

In a sandwich ELISA, the target is captured between two antibodies. It is sensitive and specific and suits larger proteins such as cytokines, growth factors and receptors. In a competitive ELISA, the target in your sample competes with a labelled version for a limited number of binding sites, so a higher signal means less target. That format suits small molecules, such as vitamins and hormones, that cannot bind two antibodies at once. The format of each kit, for example the Vitamin B12 ELISA kit, is stated in its datasheet. Ask sales@luminixhealth.com.

The kits in this collection are set up for 48 assays, for example the Human ADAMTS7 ELISA kit, 48 assays, and the Human TNFRSF1A ELISA kit, 48 assays. Assays are wells, not samples. Standards, blanks and controls take part of the plate, and most labs run samples in duplicate, so you can measure fewer samples than the number of assays. Count your standard curve and replicates before you decide how many kits you need. For larger studies, order kits from the same lot: sales@luminixhealth.com.

ELISA kits contain components with different storage needs. For the kits listed here, the product data says to store at −20 °C or +4 °C depending on the component, for example the Human NGF ELISA kit and the Mouse Hepcidin ELISA kit. Unpack them as soon as they arrive and put each component at its own temperature. Avoid repeated freezing and thawing of standards, and let reagents reach room temperature before the assay. Check the expiry date on the label. Delivery questions: sales@luminixhealth.com.

An ELISA kit contains the plate, the standards, the antibody and the substrate for one analyte, so a concentration can be read as a colour. The range of the standard curve, the sample type it was validated for, and the expiry are the specification. Mixing components from two lots is a different assay.

An antibody binds one target. A primary antibody binds the molecule of interest. A secondary antibody binds the primary and usually carries the signal, an enzyme or a dye. An ELISA kit is that binding arranged in a well so the amount of target becomes a colour. The host species, the clone, the antigen and the stated application are the specification. A name alone is not. The literature is on PubMed. Sequences of the targets are on NCBI Nucleotide. Reference material is catalogued by ATCC.

This shelf belongs to Antibodies & ELISA kits, inside Microbiology lab supplies. Luminix Health distributes this range for laboratory use.

The neighbouring shelves are Primary antibodies, Recombinant proteins & cytokines and Secondary antibodies. The category above this shelf is Antibodies & ELISA kits.

Luminix Health distributes this range from the heart of Europe. We deliver across the EU in two to five days if in stock. Standard delivery is free from €250 excl. VAT. Cold-chain goods and dangerous goods under ADR are never included in that free delivery. The terms are in the Shipping Policy. Send a quote request, contact the team, or read who we are. The full brand index is on shop by brand.

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