anti-Cardiac Actin mouse, monoclonal, AC1-20.4.2, 0.2 ml, plastic - Luminix Health
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anti-Cardiac Actin mouse, monoclonal, AC1-20.4.2, 0.2 ml, plastic - Luminix Health

anti-Cardiac Actin mouse, monoclonal, AC1-20.4.2, 0.2 ml, plastic

SKU: 1NLN.1
Volume: 0.2 ml
Value Packs: 1 piece
Price:
€109,15

Description

anti-Cardiac Actin mouse, monoclonal, AC1-20.4.2, 50 µg/ml, affinity purified (made by PROGEN), Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +4 °C

This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.

  • High epitope affinity
  • Highly specific detection of the target protein
  • Unconjugated
  • Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence

Ac1 represents an excellent marker for cardiac tissue; it discriminates fetal (cardiac) a-actin from all other actin isoforms. Fetal actin can be localized in regenerating skeletal muscle after injury (in satellite cells) and in veins of the umbelical cord. Mab Ac1-20.4.2 shows no cross reaction with other actin isoforms present in skeletal and smooth muscle, provided that stringent experimental conditions have been applied. Polypeptide reacting: specific for fetal (cardiac) isoform of actin.

Application

Immunohistochemistry (IHC) - frozen: 1:10 (5 μg/ml; include 0.5 M NaCl in all washing buffers to enhance specificity) Immunohistochemistry (IHC) - paraffin: 1:10 (5 μg/ml; microwave treatment recommended) Western Blot (WB): 1:1,000 (50 ng/ml; include 1 M NaCl in all washing buffers to enhance specificity)

Western blot analysis of human heart whole tissue lysate and with anti-Cardiac Actin antibody. Western blot analysis was performed on 20 μg human heart lysate. The PVDF membrane was blocked with 5% milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-Cardiac Actin mouse monoclonal, AC1-20.4.2 (Cat. No. 690075) was diluted in blocking buffer (antibody concentration 0.05 μg/ml) and incubated for 1 h at RT. The secondary antibody anti-mouse IgG, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.
anti-Cardiac Actin mouse monoclonal, AC1-20.4.2, liquid, purified
Not a medical device / Not an IVD product
Technical Information
Application IHC WB 
Host species mouse 
Clonality monoclonal 
Reactivity antibody cattle chicken human mouse rabbit 
Purification Affinity chromatography 
Conjugate uncojugated 
Concentration 50 µg/ml 
Isotype IgG1 
Uniprot ID AK P68034 (chicken), P68032 (human), G1STB6 (rabbit) 
Clone AC1-20.4.2 
Target cardiac a-actin 

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