anti-EP-CAM mouse monoclonal, HEA125, 0.2 ml, plastic

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anti-EP-CAM mouse monoclonal, HEA₁₂₅, 50 µg/ml, affinity purified (made by PROGEN), Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +4 °C

This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.

High epitope affinity
Highly specific detection of the target protein
Unconjugated
Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence

The Ep-CAM (HEA125) antibody recognizes the epithelial cell adhesion molecule Ep-CAM (also described as 17-1A antigen, EPG34, CD326, and TACSTD1). This antigen is widely expressed on cells of epithelial origin and tumors derived therefrom. HEA125 represents an excellent marker to discriminate epithelial from mesothelial structures. The antigen has been detected in all carcinoma types tested (18 different origins). A subset of squamous cell carcinoma is negative. The antibody can be used as alternative to the anti-EpCAM antibody clone BerEp4. Polypeptide reacting: Mr 40,000 human epithelium-specific cell surface glycoprotein (Ep-CAM). Reactivity on cultured cell lines: all carcinoma cell lines tested so far; particularly strong reaction with colon carcinoma cell lines.

Application

Immunocytochemistry (ICC): assay dependentImmunohistochemistry (IHC) - frozen: 1:10-1:50 (1-5 μg/ml) Immunohistochemistry (IHC) - paraffin: 1:10-1:50 (1-5 μg/ml; protease treatment recommended) Western Blot (WB): 1:200-1:3.000 (0.02-0.25 μg/ml)

Human head and neck squamous-cell carcinoma (HNSCC)(courtesy of J. Heß, University Hospital Heidelberg)

Mouse stomach (courtesy of J.Heß, University Hospital Heidelberg)

Western blot analysis of human HT29 cell lysate with anti-Ep-CAM antibody. Western blot analysis was performed on 20 μg of HT29 lysate. Cells were lysed with RIPA buffer. The PVDF membrane was blocked with 5% dry milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-EP-CAM mouse monoclonal, HEA125 (Cat. No. 690004) was diluted in blocking buffer (antibody concentration 0.025 μg/ml) and incubated for 1 h at RT. The secondary antibody anti-mouse IgG goat polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.

Not a medical device / Not an IVD product

Technical Information

Application	ICC/IF IHC WB
Host species	mouse
Clonality	monoclonal
Reactivity antibody	mouse
Purification	Affinity chromatography
Conjugate	uncojugated
Concentration	50 µg/ml
Isotype	IgG1
Clone	HEA125
Target	HT-29 colon carcinoma cell line

Technical Documentation (PDF)

anti-EP-CAM mouse monoclonal, HEA125, 0.2 ml, plastic

Description