anti-Keratin K19 mouse, monoclonal, Ks19.2 (Z105.6), 0.2 ml, plastic

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anti-Keratin K₁₉ mouse, monoclonal, Ks₁₉.2 (Z₁₀₅.6), 50 µg/ml, affinity purified (made by PROGEN), Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +4 °C

This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.

High epitope affinity
Highly specific detection of the target protein
Unconjugated
Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence

Ks 19.2 represents an excellent marker to discriminate glandular epithelial carcinoma from those of different origin. No reaction with hepatocellular carcinoma! Polypeptide Reacting: Mr 40,000 polypeptide (keratin K19; formerly also designated cytokeratin 19) of human glandular epithelia. The epitope has been localized on aa. 352-368 (VRADSERQNQEYQRLMD) of the alpha-helical fragment. Tumors specifically detected: all tested adenocarcinoma; cholangio carcinoma of liver; renal cell carcinoma; transitional cell carcinoma of the bladder; ovary carcinoma; squamous cell carcinoma of cervix, bronchus and lung (intermediate type); mesothelioma; carcinoid tumor of bronchus; breast carcinoma; thymoma. Reactivity on cultured cell lines: MCF-7

Application

ELISA: Assay dependentImmunocytochemistry (ICC): assay dependentImmunohistochemistry (IHC) - frozen: 1:10-1:50 (1-5 µg/ml)Immunohistochemistry (IHC) - paraffin: 1:10-1:500 (0.1-5 µg/ml; microwave treatment recommended)

Western blot analysis of human MCF-7 cell lysate with anti-Keratin K19 antibody. Western blot analysis was performed on 10 μg or 5 μg of MCF-7 lysate. Cells were lysed in PBS by homogenization. The PVDF membrane was blocked with 5% dry milk in PBST for 1 h at RT. The primary antibody anti-Keratin K19 mouse monoclonal, Ks19.2 (Z105.6; Cat. No. 690029) was diluted in blocking buffer (antibody concentration 0.1 μg/ml) and incubated for 1 h at RT. The secondary antibody goat anti-mouse IgG polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.

Human head and neck squamous-cell carcinoma (HNSCC) (courtesy of J. Heß, University)

Not a medical device / Not an IVD product

Technical Information

Application	ELISA ICC/IF IHC WB
Host species	mouse
Clonality	monoclonal
Reactivity antibody	rat cattle human rabbit
Purification	Affinity chromatography
Conjugate	uncojugated
Concentration	50 µg/ml
Isotype	IgG2b
Uniprot ID AK	P08728 (bovine), P08727 (human), Q63279 (rat)
Clone	Ks19.2 (Z105.6)
Target	Keratin K19

Technical Documentation (PDF)

anti-Keratin K19 mouse, monoclonal, Ks19.2 (Z105.6), 0.2 ml, plastic

Description