anti-Perilipin 2 (N-terminus) mouse, monoclonal, AP125, 0.2 ml, plastic - Luminix Health
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anti-Perilipin 2 (N-terminus) mouse, monoclonal, AP125, 0.2 ml, plastic - Luminix Health

anti-Perilipin 2 (N-terminus) mouse, monoclonal, AP125, 0.2 ml, plastic

SKU: 1NP4.1
Volume: 0.2 ml
Value Packs: 1 piece
Price:
€130,65

Description

anti-Perilipin 2 (N-terminus) mouse, monoclonal, AP125, 50 µg/ml, affinity purified (made by PROGEN), Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +4 °C

This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.

  • High epitope affinity
  • Highly specific detection of the target protein
  • Unconjugated
  • Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence

Perilipin 2/Adipophilin/ADRP/PLIN2 is a ubiquitous component of lipid droplets. It has been found in milk fat globule membranes and on the surface of lipid droplets in various cultured cell lines; inducible by etomoxir. Enhanced expression of Perilipin 2/Adipophilin/ADRP/PLIN2 is a useful marker for pathologies characterized by increased lipid droplet accumulation. Such diseases include atheroma, steatosis, obesity and certain cases of liposarcoma. It also seems to be a potent marker for atherosclerosis. ADRP can also be used to study virus entry via lipid droplets. Polypeptide reacting: Perilipin 2/Adipophilin/ADRP/PLIN2, MW 48,100 (calculated from aa sequence data); apparent Mr 52,000 (after SDS-PAGE); pI 6.72. Immunolocalization: Perilipin 2/Adipophilin/ADRP/PLIN2 is positively detected in the glandular cells of lactating mammary gland (ductal cells are negative), zona fasciculata of the adrenal gland, Sertoli cells of the testis, and in fat-accumulating hepatocytes of alcoholic cirrhotic fatty liver; adipocytes are negative. Also positively stained are lipid-storing CD 68-positive macrophages. Tested cultured cell lines: PLC, MDCK.

Application

Immunohistochemistry (IHC) - frozen: 1:10-1:100 (0.5-5 μg/ml)Immunohistochemistry (IHC) - paraffin: 1:10-1:100 (0,5-5 μg/ml; microwave treatment recommended)Western Blot (WB): 1:50-1:100 (0.5-1 μg/ml)

Western blot analysis of HepG2 cell lysate with anti-Perilipin 2 antibody. Western blot analysis was performed on 15 μg HepG2 lysate and 15 μg HepG2 + OA lysate. Cells were previously treated with 400 μM oleic acid (OA) if indicated. Cells were lysed in RIPA buffer. The PVDF membrane was blocked with 5% dry milk in PBST for 1 h at RT. The primary antibody anti-Perilipin 2 (N-terminus) mouse monoclonal, AP125 (Cat. No. 690102) was diluted in blocking buffer (antibody concentration 0.5 μg/ml) and incubated at 4°C over-night. The secondary antibody goat anti-mouse IgG polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.
IHC of human skin (courtesy of J.Heß, University Hospital Heidelberg)
IHC of rat stomach (courtesy of J. Heß, University Hospital Heidelberg)
Not a medical device / Not an IVD product
Technical Information
Application IHC WB 
Host species mouse 
Clonality monoclonal 
Reactivity antibody dog human rat 
Purification Affinity chromatography 
Conjugate uncojugated 
Concentration 50 µg/ml 
Isotype IgG1 
Uniprot ID AK A0A5F4CRI5 (dog, canis familiaris), Q99541 (human), Q5U2U5 (rat) 
Clone AP125 
Target Perilipin 2/Adipophilin/ADRP/PLIN2 

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