Description
EC 250/2 NUCLEODUR C18 Isis, 5 µm is an EC HPLC separation column for high-performance liquid chromatography. It offers a column length of 250 mm with an internal diameter of 2 mm and NUCLEODUR C18 Isis stationary phase at a particle size of 5 µm. The longer bed and narrow internal diameter suit analytical HPLC setups where this geometry is specified. Pack size is one column per pack for single-column laboratory use. Length, internal diameter and particle size are fully defined to support method documentation and column selection. The NUCLEODUR C18 Isis designation denotes the bonded phase chemistry of this EC column from manufacturer Macherey-Nagel. In the HPLC chromatography columns category, the 250 mm × 2 mm format is chosen when extended length and 5 µm NUCLEODUR C18 Isis packing are required. EC hardware dimensions allow integration into conventional HPLC instrument configurations. The combination of 250 mm length, 2 mm internal diameter and 5 µm particle size provides a fixed column format for chromatographic separation work in research and analytical laboratories.
This article is part of HPLC columns, in HPLC columns & accessories. See also HPLC guard columns.
Key features
- Pack size: 1 pc
- Product type: EC HPLC separation column
- Column length: 250 mm
- Internal diameter: 2 mm
- Particle size: 5 µm
- EAN: 4046681062810
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Questions about EC 250/2 NUCLEODUR C18 Isis, 5 µm
What is this article?
EC 250/2 NUCLEODUR C18 Isis, 5 µm is the article on this page. Luminix Health distributes Macherey-Nagel for laboratory use. It sits in HPLC columns, inside HPLC columns & accessories, in Chromatography equipment.
An HPLC column is specified by the stationary phase, the particle size, the length and the internal diameter. A C18, 5 µm, 150 × 4.6 mm column is a different article from a C18, 3 µm, 100 × 2.1 mm column. The smaller bore belongs on a lower flow. A column has a direction and a pH range.
An HPLC column is a tube packed, or coated, with a stationary phase. The sample is carried through it by a liquid, and the compounds leave at different times. The phase, the particle size, the length and the internal diameter decide the separation. A guard column is a short sacrificial piece in front of the analytical column. Fittings and tubing have a bore that either preserves the peak or smears it. Compound names follow IUPAC. Data are on PubChem. Method grades often cite the European Pharmacopoeia or USP.
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