Description
EC 250/2 NUCLEODUR HILIC, 5 µm is an EC HPLC separation column designed for hydrophilic interaction liquid chromatography workflows. The column combines a 250 mm bed length with a 2 mm internal diameter and a 5 µm NUCLEODUR HILIC stationary phase, giving a compact analytical format suited to method development and routine separations of polar analytes. Supplied as a single column, it fits standard HPLC systems that accept EC-format hardware and supports reproducible gradient or isocratic HILIC operation. The NUCLEODUR HILIC chemistry provides a dedicated polar surface for retention of hydrophilic compounds that are poorly retained under reversed-phase conditions, while the 5 µm particle size balances efficiency with practical back-pressure on conventional pumps. Dimensions of 250 mm length and 2 mm ID keep solvent consumption moderate and are convenient for laboratories working with limited sample volumes. This EC 250/2 configuration from manufacturer Macherey-Nagel is intended for analytical HPLC laboratories performing HILIC separations in pharmaceutical, biochemical, or general chemical analysis. Each unit is packed individually for straightforward installation and inventory control.
This article is part of HPLC columns, in HPLC columns & accessories. See also HPLC guard columns.
Key features
- Pack size: 1 pc
- Column type: EC HPLC separation column
- Stationary phase: NUCLEODUR HILIC
- Particle size: 5 µm
- Column length: 250 mm
- EAN: 4046681061264
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Questions about EC 250/2 NUCLEODUR HILIC, 5 µm
What is this article?
EC 250/2 NUCLEODUR HILIC, 5 µm is the article on this page. Luminix Health distributes Macherey-Nagel for laboratory use. It sits in HPLC columns, inside HPLC columns & accessories, in Chromatography equipment.
An HPLC column is specified by the stationary phase, the particle size, the length and the internal diameter. A C18, 5 µm, 150 × 4.6 mm column is a different article from a C18, 3 µm, 100 × 2 mm column. The smaller bore belongs on a lower flow. A column has a direction and a pH range.
An HPLC column is a tube packed, or coated, with a stationary phase. The sample is carried through it by a liquid, and the compounds leave at different times. The phase, the particle size, the length and the internal diameter decide the separation. A guard column is a short sacrificial piece in front of the analytical column. Fittings and tubing have a bore that either preserves the peak or smears it. Compound names follow IUPAC. Data are on PubChem. Method grades often cite the European Pharmacopoeia or USP.
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