Description
EC 250/4 NUCLEODUR C18 Gravity, 5 µm is an analytical HPLC separation column designed for reversed-phase liquid chromatography across pharmaceutical, chemical, and life-science laboratories. It combines a column length of 250 mm with an internal diameter of 4 mm and is packed with 5 µm NUCLEODUR C18 Gravity particles offering a hydrophobic C18 bonded phase for efficient separation of non-polar to moderately polar analytes. This EC HPLC column from manufacturer Macherey-Nagel is delivered as a single unit and integrates with conventional analytical HPLC instrumentation. The Gravity C18 chemistry is suitable for method development, routine quantification, and impurity analysis where reproducible retention and good peak symmetry matter. Compatible mobile phases typically include water–acetonitrile or water–methanol mixtures with appropriate buffers, applied according to validated laboratory procedures. The 250 mm length supports solid resolving power for multi-component samples, while the 4 mm internal diameter balances sensitivity, loadability, and practical flow rates for everyday analytical work. Supplied one column per pack, it is ready for installation or stock replacement in quality-control, research, and process-support environments that depend on dependable reversed-phase HPLC columns.
This article is part of HPLC columns, in HPLC columns & accessories. See also HPLC guard columns.
Key features
- Pack size: 1 pc
- Column type: EC HPLC separation column
- Stationary phase: NUCLEODUR C18 Gravity
- Particle size: 5 µm
- Column length: 250 mm
- EAN: 4046681065019
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Questions about EC 250/4 NUCLEODUR C18 Gravity, 5 µm
What is this article?
EC 250/4 NUCLEODUR C18 Gravity, 5 µm is the article on this page. Luminix Health distributes Macherey-Nagel for laboratory use. It sits in HPLC columns, inside HPLC columns & accessories, in Chromatography equipment.
An HPLC column is specified by the stationary phase, the particle size, the length and the internal diameter. A C18, 5 µm, 150 × 4.6 mm column is a different article from a C18, 3 µm, 100 × 2 mm column. The smaller bore belongs on a lower flow. A column has a direction and a pH range.
An HPLC column is a tube packed, or coated, with a stationary phase. The sample is carried through it by a liquid, and the compounds leave at different times. The phase, the particle size, the length and the internal diameter decide the separation. A guard column is a short sacrificial piece in front of the analytical column. Fittings and tubing have a bore that either preserves the peak or smears it. Compound names follow IUPAC. Data are on PubChem. Method grades often cite the European Pharmacopoeia or USP.
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The category is HPLC columns & accessories. The sub-category is HPLC columns. The catalogue is Chromatography equipment.
Shelves beside this one are HPLC fittings, tubing & inline filters, HPLC guard columns and HPLC accessories.
What should I check before I order?
When this page shows a safety data sheet, use that manufacturer file. Hazard text does not belong in the sales description. For a pack size, a grade or a certificate that is not on the page, send a quote request or contact the team.
How does ordering and shipping work?
Luminix Health distributes this article from the heart of Europe. Delivery across Europe, two to five days when in stock. Standard delivery is free from €250 excl. VAT. Cold-chain goods and dangerous goods under ADR are never included in that free delivery. The terms are in the Shipping Policy. The full brand index is on shop by brand. Who we are is on about us.
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The seven catalogues are Labware, Lab chemicals, Lab instruments, Lab consumables, Chromatography equipment, Lab safety equipment and Life Science & Microbiology.
