Description
EC 250/4 NUCLEOSIL 100-5 C8 is an EC HPLC separation column for analytical liquid chromatography applications. The column has a length of 250 mm and an internal diameter of 4 mm, and is packed with NUCLEOSIL 100-5 C8 stationary phase—an octyl-modified silica suited to reversed-phase separations that benefit from somewhat lower hydrophobicity than C18. Supplied as one piece, it is appropriate for routine analyses and method work on HPLC systems configured for 4 mm ID columns. The C8 chemistry supports separation of moderately polar to non-polar compounds where adjusted retention relative to C18 is desired. This column from manufacturer Macherey-Nagel forms part of the NUCLEOSIL EC portfolio used in research, quality control, and analytical laboratories. Approximate net weight is 0,28 kg per unit. Operate according to standard HPLC practice for C8 reversed-phase columns of this length, diameter, and particle specification.
This article is part of HPLC columns, in HPLC columns & accessories. See also HPLC guard columns.
Key features
- Pack size: 1 pc
- Product type: EC HPLC separation column
- Stationary phase: NUCLEOSIL 100-5 C8
- Length: 250 mm
- Internal diameter: 4 mm
- EAN: 4046681082900
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Questions about EC 250/4 NUCLEOSIL 100-5 C8
What is this article?
EC 250/4 NUCLEOSIL 100-5 C8 is the article on this page. Luminix Health distributes Macherey-Nagel for laboratory use. It sits in HPLC columns, inside HPLC columns & accessories, in Chromatography equipment.
An HPLC column is specified by the stationary phase, the particle size, the length and the internal diameter. A C18, 5 µm, 150 × 4.6 mm column is a different article from a C18, 3 µm, 100 × 2.1 mm column. The smaller bore belongs on a lower flow. A column has a direction and a pH range.
An HPLC column is a tube packed, or coated, with a stationary phase. The sample is carried through it by a liquid, and the compounds leave at different times. The phase, the particle size, the length and the internal diameter decide the separation. A guard column is a short sacrificial piece in front of the analytical column. Fittings and tubing have a bore that either preserves the peak or smears it. Compound names follow IUPAC. Data are on PubChem. Method grades often cite the European Pharmacopoeia or USP.
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