Description
EC 4/2 NUCLEODUR HILIC, 5 µm is an HPLC guard column for hydrophilic interaction liquid chromatography. It has a length of 4 mm, an internal diameter of 2 mm and a particle size of 5 µm, and is intended for main columns with a matching 2 mm internal diameter. The NUCLEODUR HILIC phase supports retention of polar analytes while the short guard bed traps particulates and strongly retained sample components before they reach the analytical column. Supplied in packs of three pieces from manufacturer Macherey-Nagel, these guard columns require the Column Protection System guard-column holder (REF 718966) for correct installation. The compact EC 4/2 format fits narrow-bore HILIC workflows used in pharmaceutical, environmental and food analysis. When properly mounted, the guard column helps preserve chromatographic efficiency and extends the service life of the main column under the aqueous–organic mobile-phase conditions typical of HILIC separations, making it a practical protective element for routine and method-development work.
This article is part of HPLC columns, in HPLC columns & accessories. See also HPLC guard columns.
Key features
- Pack size: 3 pc(s)
- Type: HPLC guard column EC 4/2
- Phase: NUCLEODUR HILIC
- Particle size: 5 µm
- Length: 4 mm
- EAN: 4046681057236
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Questions about EC 4/2 NUCLEODUR HILIC, 5 µm
What is this article?
EC 4/2 NUCLEODUR HILIC, 5 µm is the article on this page. Luminix Health distributes Macherey-Nagel for laboratory use. It sits in HPLC guard columns, inside HPLC columns & accessories, in Chromatography equipment.
A guard column is a short column of the same phase as the analytical column, placed in front of it to catch particles and compounds that would stick forever. It is meant to be replaced. A guard of a different phase changes the separation. The holder and the cartridge are often separate parts.
An HPLC column is a tube packed, or coated, with a stationary phase. The sample is carried through it by a liquid, and the compounds leave at different times. The phase, the particle size, the length and the internal diameter decide the separation. A guard column is a short sacrificial piece in front of the analytical column. Fittings and tubing have a bore that either preserves the peak or smears it. Compound names follow IUPAC. Data are on PubChem. Method grades often cite the European Pharmacopoeia or USP.
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Shelves beside this one are HPLC columns, HPLC fittings, tubing & inline filters and HPLC accessories.
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