Description
Ethidium bromide solution 0.5 % is a ready-to-use aqueous solution at 5 mg/ml, It is applied in horizontal agarose electrophoresis workflows to stain DNA and RNA in gels after or during separation. Physical data include a density of 1 g/cm³ and a boiling point of approximately 100 °C, consistent with an aqueous preparation. The solution is available from manufacturer Carl ROTH in a bottle format that supports direct use without weighing solid dye, streamlining routine gel staining. size suits research groups, service labs, and teaching environments that run regular agarose electrophoresis. Typical use falls within electrophoresis applications focused on visualisation of nucleic acids in agarose gels. Ready-to-use presentation reduces preparation steps compared with dissolving powder stocks and helps standardise stain concentration at the bench. Users should follow institutional safety rules and consult the accompanying safety documentation before handling. Integration into existing staining or post-stain washing protocols remains under the laboratory’s validated methods, with volumes adjusted to gel size and detection setup.
This article is part of Fluorescent dyes & labels, in Dyes, stains & indicators.
Key features
- Pack sizes: 15 ml, 75 ml
- Density: 1 g/cm³
- Boiling point: ~100 °C
- Concentration: 0.5 % (5 mg/ml)
- Form: Aqueous solution, ready-to-use
- Technical Information Sheet:
- Safety Data Sheet:
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Questions about Ethidium bromide solution 0.5 %, bottle, aqueous solution (5 mg/ml), 15 ml
What is this article?
Ethidium bromide solution 0.5 %, bottle, aqueous solution (5 mg/ml), 15 ml is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Fluorescent dyes & labels, inside Dyes, stains & indicators, in Lab chemicals.
A fluorescent dye absorbs at one wavelength and emits at a longer one. As a label it is attached to a protein, a nucleic acid or a cell. Excitation and emission maxima, and the reactive group if it must conjugate, are the specification. The colour name does not tell you the wavelengths.
A dye or a stain binds, or changes colour, so that something invisible becomes visible. An indicator changes at a pH or a redox point. A fluorescent dye absorbs one wavelength and emits another, which is how a microscope or a plate reader picks it out. The excitation and emission, or the transition interval, are the specification. The colour name on the label is not. Structures are on PubChem under IUPAC names. Methods are in the PubMed literature.
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The category is Dyes, stains & indicators. The sub-category is Fluorescent dyes & labels. The catalogue is Lab chemicals.
Shelves beside this one are Microscopy stains & staining solutions and Dyes & pigments.
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