Description
HEPES sodium salt is a zwitterionic buffer salt offered as PUFFERAN® grade with a minimum purity of 99 %, CAS number 75277-39-3 and empirical formula C₈H₁₇N₂O₄SNa. The molar mass is 260,3 g/mol and the density is 1,51 g/cm³. This sodium salt form is widely used to prepare biological and biochemical buffer systems near physiological pH for cell culture media, enzyme assays and electrophoresis. Laboratories select HEPES sodium salt when stable buffering capacity and low metal-ion interference are required in life-science and biophysical workflows. The PUFFERAN® specification and convenient pack size support method development, routine media preparation and research protocols that depend on a well-characterised Good’s buffer component. Its defined composition enables reproducible pH control in aqueous systems used throughout molecular biology and biochemistry.
This article is part of Biological buffers (Tris, HEPES, MOPS), in Buffers & pH solutions.
Key features
- Appearance: white powder
- Assay (titr., dry substance): ≥99,0 %
- Loss on drying: ≤1,5 %
- pH value (1 M in H2O): 10,0-12,0
- 260 nm: ≤0,2
- 280 nm: ≤0,2
- CAS number: 75277-39-3
- Pack sizes: 25 g, 100 g
- EG nr.: 278-169-7
- Purity: PUFFERAN® min. 99 %
- Empirical formula: C₈H₁₇N₂O₄SNa
- Molar mass: 260,3 g/mol
- Technical Information Sheet:
- Safety Data Sheet:
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Questions about HEPES sodium salt, PUFFERAN®, min. 99 %, 25 g, plastic
What is this article?
HEPES sodium salt, PUFFERAN®, min. 99 %, 25 g, plastic is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Biological buffers (Tris, HEPES, MOPS), inside Buffers & pH solutions, in Lab chemicals.
Tris, HEPES, MOPS and their relatives hold pH in a range that cells and proteins tolerate, with a pKa near the working pH. The useful range is roughly one unit either side of the pKa. A phosphate buffer and Tris are not interchangeable in a method that names one of them, because the ion itself has effects.
A buffer resists a change in pH when a little acid or base is added. It does that because a weak acid and its conjugate base are both present. A pH buffer used to calibrate a meter has a stated value at a stated temperature. A biological buffer such as Tris or HEPES is chosen because it holds the pH of a living system and does not poison it. Conductivity standards are the sister articles for a conductivity meter. The ions are on PubChem. Electrode calibration practice sits with EURAMET and BELAC.
Which category should I open next?
The category is Buffers & pH solutions. The sub-category is Biological buffers (Tris, HEPES, MOPS). The catalogue is Lab chemicals.
Shelves beside this one are pH buffers & conductivity standards and Buffers (other).
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