Description
Ribonuclease H is a hydrolase enzyme preparation supplied at 5 U/µl for molecular biology applications. Identified by CAS number 9001-99-4 and EG number 232-646-6, it is used in nucleic-acid workflows that require selective cleavage of RNA within RNA–DNA hybrids, including reverse-transcription clean-up and related cloning steps. The enzyme has an approximate molar mass of 13 700 g/mol and a density of 1,18 g/cm³, giving clear physical reference values for laboratory handling. The defined activity concentration supports reproducible addition to established molecular protocols. This preparation is available from manufacturer Carl ROTH in the enzymes and substrates range for hydrolase work. Laboratories performing hybrid-duplex processing can integrate the reagent into standard buffer systems and incubation schemes without excess working volume. The compact fill is practical for cold-bench organisation while maintaining the activity level expected for routine molecular biology procedures that depend on controlled RNase H function.
This article is part of Enzymes & substrates, in Biochemicals & reagents.
Key features
- Purity: ≥90 %
- Concentration: 5 U/µl
- DNases (exo-and endonucleases): no
- Pack sizes: 50 µl, 250 µl
- CAS number: 9001-99-4
- Technical Information Sheet:
- Safety Data Sheet:
Compliance & Safety Information
DangerHazard Statements (H-Phrases)
Precautionary Statements (P-Phrases)
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Questions about Ribonuclease H, 5 U/µl, for Molecular Biology, 50 µl, plastic
What is this article?
Ribonuclease H, 5 U/µl, for Molecular Biology, 50 µl, plastic is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Enzymes & substrates, inside Biochemicals & reagents, in Microbiology lab supplies.
An enzyme is sold by activity, in units defined by a stated reaction, not only by milligrams. Source and specific activity are the specification. A substrate is the molecule that enzyme converts, often a chromogenic one so the reaction can be seen. Units from two suppliers are comparable only when the definition matches.
A biochemical is a molecule of life, sold as a reagent: an amino acid, a sugar, a nucleotide, a lipid, an enzyme, an antibiotic. The difference from a bulk chemical is that the use is a biological one, and the impurities that matter are the ones that change that use. An enzyme is specified by activity, not only by mass. An antibiotic is specified by potency. Papers are on PubMed. Structures are on PubChem. Strains and lines that consume these reagents are at ATCC.
Which category should I open next?
The category is Biochemicals & reagents. The sub-category is Enzymes & substrates. The catalogue is Microbiology lab supplies.
Shelves beside this one are Amino acids & peptides, Carbohydrates & sugars, Coenzymes, nucleotides & vitamins, Biochemicals (other), Antibiotics & antimycotics, Lipids, steroids & fatty acids and Detergents & surfactants.
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The seven catalogues are Labware, Lab chemicals, Lab instruments, Lab consumables, Chromatography equipment, Lab safety equipment and Life Science & Microbiology.