Description
ROTI®Garose-His/Ni Columns are ready-to-use affinity chromatography columns intended for biochemistry applications in research and analytical laboratories. Supplied as a pack of 8 units in cardboard packaging, they offer a practical format for repeated purification runs or parallel sample processing. Physical characteristics include a density of 0,85 to 0,95 g/cm³, a flash point above 50 °C and a boiling point above 80 °C. These columns sit within the bio-chromatography and affinity chromatography field and are suited to separations that employ nickel-based His-binding media. Available from manufacturer Carl ROTH, ROTI®Garose-His/Ni Columns help laboratories set up immobilized metal affinity chromatography with a pre-packed format that reduces resin-packing steps and supports consistent bed geometry from run to run. They fit workflows for isolation and enrichment of target biomolecules where His/Ni chemistry is specified. The combination of defined physical properties, convenient multi-unit packaging and ready-to-use presentation makes the columns a practical choice for biochemistry groups performing routine affinity chromatography and related protein purification tasks.
This article is part of Flash, empty & glass chromatography columns, in SPE & sample preparation.
Key features
- Particle size: 50-150 µm
- Beads (cross-linked agarose): 6 %
- Ligand: IDA
- Metal ion: nickel
- Binding capacity (DHAK-6xHis): 117 mg/ml
- Stabilizer: 20 % ethanol
- Pack sizes: 8 pc(s), 5 pc(s)
- Density: 0,85–0,95 g/cm³
- Flash point: >50 °C
- Boiling point: >80 °C
- Technical Information Sheet:
- Safety Data Sheet:
Compliance & Safety Information
WarningHazard Statements (H-Phrases)
Precautionary Statements (P-Phrases)
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Questions about ROTI®Garose-His/Ni Columns, for biochemistry, 8 pieces, cardboard
What is this article?
ROTI®Garose-His/Ni Columns, for biochemistry, 8 pieces, cardboard is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Flash, empty & glass chromatography columns, inside SPE & sample preparation, in Chromatography equipment.
A flash column or an empty glass column is the tube a separation is packed or run in, under gravity or under air pressure. Diameter and length, and whether it arrives prepacked, are the choices. An empty column still needs a frit and a sorbent.
Solid-phase extraction holds a compound on a sorbent while the rest of the sample is washed away, then releases it in a small volume. The sorbent, the bed mass and the solvents are the method. Silica, ion exchangers and polymer phases do different jobs. A flash column is the same idea scaled toward purification rather than trace cleanup. The molecules are on PubChem, named by IUPAC.
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The category is SPE & sample preparation. The sub-category is Flash, empty & glass chromatography columns. The catalogue is Chromatography equipment.
Shelves beside this one are SPE cartridges & columns and Sorbents, silica gel & ion exchangers.
What should I check before I order?
The technical information on this page is the manufacturer's sheet. Read that, not a rewritten specification.
When this page shows a safety data sheet, use that manufacturer file. Hazard text does not belong in the sales description. For a pack size, a grade or a certificate that is not on the page, send a quote request or contact the team.
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