Description
ROTI®Garose-His uncharged HPBeads are biochemistry-grade chromatography beads supplied ready for laboratories that charge the matrix themselves or require an unloaded His-binding support. is sized for method setup, screening and moderate-scale affinity work. According to the manufacturer description, density lies between 0,85 and 0,95 g/cm³, with a flash point above 50 °C, a boiling point above 80 °C and UN number 1170. These uncharged HPBeads from manufacturer Carl ROTH form part of the affinity chromatography offering within bio-chromatography applications. Users typically load a suitable metal ion before capturing histidine-tagged proteins, or deploy the base matrix where an uncharged agarose-type bead is preferred. The defined density range and thermal data help with slurry handling, column packing and solvent selection. Integrate the medium into standard His-tag purification schemes, following equilibration, binding and elution conditions appropriate to the chosen metal and buffer system, and observe the handling information given in the accompanying documentation.
This article is part of Sorbents, silica gel & ion exchangers, in SPE & sample preparation.
Key features
- Particle size: 50-150 µm
- Beads (cross-linked agarose): 6 %
- Ligand: IDA
- Metal ion: uncharged
- Loading capacity (gel) (Ni2+): ~20 µmol/ml
- Size exclusion limit (Da): ~4x106
- Ethanol: 20 %
- Pack sizes: 25 ml, 100 ml
- Density: 0,85 to 0,95 g/cm³
- Flash point: >50 °C
- Boiling point: >80 °C
- Storage temperature: +4 °C
- Technical Information Sheet:
- Safety Data Sheet:
Compliance & Safety Information
WarningHazard Statements (H-Phrases)
Precautionary Statements (P-Phrases)
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Questions about ROTI®Garose-His uncharged HPBeads, for biochemistry, 25 ml, plastic
What is this article?
ROTI®Garose-His uncharged HPBeads, for biochemistry, 25 ml, plastic is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Sorbents, silica gel & ion exchangers, inside SPE & sample preparation, in Chromatography equipment.
Loose sorbent, silica gel and ion-exchange resin are the phases bought in bulk to pack a column or to stir into a solution. Particle size and activity, or the ion-exchange capacity, are the specification. A TLC silica and a flash silica of different particle sizes are not the same separation.
Solid-phase extraction holds a compound on a sorbent while the rest of the sample is washed away, then releases it in a small volume. The sorbent, the bed mass and the solvents are the method. Silica, ion exchangers and polymer phases do different jobs. A flash column is the same idea scaled toward purification rather than trace cleanup. The molecules are on PubChem, named by IUPAC.
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The category is SPE & sample preparation. The sub-category is Sorbents, silica gel & ion exchangers. The catalogue is Chromatography equipment.
Shelves beside this one are SPE cartridges & columns and Flash, empty & glass chromatography columns.
What should I check before I order?
The technical information on this page is the manufacturer's sheet. Read that, not a rewritten specification.
When this page shows a safety data sheet, use that manufacturer file. Hazard text does not belong in the sales description. For a pack size, a grade or a certificate that is not on the page, send a quote request or contact the team.
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Luminix Health distributes this article from the heart of Europe. Delivery across Europe, two to five days when in stock. Standard delivery is free from €250 excl. VAT. Cold-chain goods and dangerous goods under ADR are never included in that free delivery. The terms are in the Shipping Policy. The full brand index is on shop by brand. Who we are is on about us.
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