Description
ROTI®Garose-Protein A Beads, for biochemistry, Density (D) 0,85 to 0,95 g/cm³, Flash point (flp) >50 °C, Boiling point (bp) >80 °C, Storage temp. +4 °C, UN-Nr. 1170
For rapid and simple purification of antibodies with reasonable effort, affinity chromatography still is the first choice in most cases. ROTI®Garose-Protein A and G Beads offer the perfect solution for your specific application under medium pressure (MPLC, FPLC), if rapid flow rates are desired or if big samples volumes have to be purified.
- Rapid one-step purification of immunoglobulins from cell lysates and biological solutions
- High yield of very pure immunoglobulins
- Easy elution and regeneration
- Covalent binding of protein G in high ligand density
The matrix of ROTI®Garose-Protein A and G Beads consists of crosslinked and beaded 4 % agarose, coated and covalently coupled with protein A from Staphylococcus aureus and protein G from Streptococcus, respectively.The recombinant protein A (recA) shares IgG binding properties with natural protein A of S. aureus Cowan strain I. It has high affinity for IgG from a variety of different mammalian species, also binding some populations of IgA and IgM.Binding domains specific for albumin as well as cell walls and cell membranes have been removed in the recombinant protein G, in order to ensure the maximum specific IgG binding capacity. It has high affinity for IgG from a variety of different mammalian species, also binding IgG3 with high affinity.
Most immunoglobulins may be eluted in 100 mM glycin or citric acid buffer (pH 3.0). ROTI®Garose-Protein A and G Beads may be regenerated, making them very cost-effective.May not be autoclaved.
Protein A coated agarose beads for low pressure affinity chromatography. High-performance affinity resin for antibody purification.50 % bead suspension in 20 % ethanol.
- Binding capacity (human IgG) of approx. 25 mg per ml matrix
- Superior affinity for IgG from various mammalian species
- Binding of some populations IgA and IgM
- For batch mode, gravity flow, MPLC und FPLC
Isolation of antibodies, in affinity column chromatography or in batch procedures.
The matrix of ROTI®Garose Protein A Beads consists of cross-linked and beaded 4 % agarose, coated and covalently coupled with protein A from Staphylococcus aureus.High binding affinity to IgGs of many different mammalian species, binding of some populations IgA and IgM.
| Instructions for use, addition | Bead suspension, binding capacity ca. 25 mg/ml Suspension (human IgG) |