Description
SDS-PAGE sample buffer concentrated is a pharmacopoeia-quality laboratory reagent for preparing protein samples prior to sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE). It is specified as Reag. Ph. Eur. in accordance with European Pharmacopoeia Chapter 4.1.1 under reagent number 1115000, placing it among reagents and volumetric standard solutions of pharmacopoeial quality. The concentrated buffer is offered in a 125 ml pack size. Density is 1,04 g/cm³ and the boiling point is 100 °C, values that support dispensing, typical heating of electrophoresis samples, and laboratory records. As a concentrated SDS-PAGE sample buffer, it is combined with protein solutions so that polypeptides can be denatured and coated with detergent before loading onto polyacrylamide gels; the concentrated format allows the working ratio to be set in the laboratory. This reagent from manufacturer Carl ROTH belongs to the Ph. Eur. Reagents 4.1.1 group used in analytical and biochemical laboratories that require pharmacopoeia-referenced chemicals. The 125 ml unit, corresponding to a net weight of 0,300 kg, is suited to routine sample preparation for SDS-PAGE.
Key features
- Pack size: 125 ml
- Density: 1,04 g/cm³
- Boiling point: 100 °C
- Quality: Reag. Ph. Eur.
- Ph. Eur. chapter: 4.1.1
- UNSPSC: 41116105
- Technical Information Sheet:
- Safety Data Sheet:
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Questions about SDS-PAGE sample buffer concentrated
What is this article?
SDS-PAGE sample buffer concentrated is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Biological buffers (Tris, HEPES, MOPS), inside Buffers & pH solutions, in Lab chemicals.
Tris, HEPES, MOPS and their relatives hold pH in a range that cells and proteins tolerate, with a pKa near the working pH. The useful range is roughly one unit either side of the pKa. A phosphate buffer and Tris are not interchangeable in a method that names one of them, because the ion itself has effects.
A buffer resists a change in pH when a little acid or base is added. It does that because a weak acid and its conjugate base are both present. A pH buffer used to calibrate a meter has a stated value at a stated temperature. A biological buffer such as Tris or HEPES is chosen because it holds the pH of a living system and does not poison it. Conductivity standards are the sister articles for a conductivity meter. The ions are on PubChem. Electrode calibration practice sits with EURAMET and BELAC.
Which category should I open next?
The category is Buffers & pH solutions. The sub-category is Biological buffers (Tris, HEPES, MOPS). The catalogue is Lab chemicals.
Shelves beside this one are pH buffers & conductivity standards and Buffers (other).
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When this page shows a safety data sheet, use that manufacturer file. Hazard text does not belong in the sales description. For a pack size, a grade or a certificate that is not on the page, send a quote request or contact the team.
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Luminix Health distributes this article from the heart of Europe. Delivery across Europe, two to five days when in stock. Standard delivery is free from €250 excl. VAT. Cold-chain goods and dangerous goods under ADR are never included in that free delivery. The terms are in the Shipping Policy. The full brand index is on shop by brand. Who we are is on about us.
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The seven catalogues are Labware, Lab chemicals, Lab instruments, Lab consumables, Chromatography equipment, Lab safety equipment and Life Science & Microbiology.