DNA-Marker short-run 1, DNA-ladder (lyophil.), 50 µg, plastic

Catalog nr. 0146.1
Carl Roth

Volume: 50 µg
Value Packs: 1 piece
Price:
Sale price€104,85
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Description

DNA-Marker short-run 1 is a DNA ladder supplied lyophilised at 50 µg in a plastic pack, together with gel loading buffer, and is not pre-dyed. It is intended for horizontal agarose electrophoresis and the separation of nucleic acids in agarose gels, where a short-run marker is required to size DNA fragments. The lyophilised ladder format allows reconstitution according to laboratory practice, while the accompanying loading buffer supports sample application on agarose gels. Because the marker is not pre-dyed, staining or imaging follows the usual laboratory nucleic-acid visualisation method rather than a built-in dye track. This DNA-Marker short-run 1 from manufacturer Carl ROTH is catalogued for electrophoresis applications that need a compact ladder for short runs. The 50 µg quantity is presented in a small plastic unit typical of DNA markers used in molecular-biology workflows. A listed pack weight of 0,012 kg corresponds to this compact presentation. Users working with agarose gels can load the short-run ladder alongside experimental samples to compare fragment migration.

This article is part of Protein extraction, purification & markers, in Protein biology & Western blot.

Key features

  • Pack size: 50 µg plastic
  • Storage temperature: -20 °C
  • Form: DNA ladder (lyophilised)
  • Dye status: Not pre-dyed
  • Included: Gel loading buffer
  • Technical Information Sheet: PDF
  • Safety Data Sheet: PDF

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Questions about DNA-Marker short-run 1, DNA-ladder (lyophil.), 50 µg, plastic

What is this article?

DNA-Marker short-run 1, DNA-ladder (lyophil.), 50 µg, plastic is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Protein extraction, purification & markers, inside Protein biology & Western blot, in Microbiology lab supplies.

Extraction reagents break the cell and keep the protein soluble. Purification resins and columns, often an affinity tag such as His, pull one protein out. A marker is a set of known masses run in the next lane. The mass range of the marker has to cover the protein being sought.

Protein work separates a mixture, then asks which protein is which. Extraction and purification take the protein out of the cell. A marker shows the masses. A Western blot moves the separated proteins onto a membrane and probes them with an antibody. The membrane, nitrocellulose or PVDF, has to bind the protein and survive the washes. Antibodies and methods are in PubMed. Reference material is at ATCC.

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The technical information on this page is the manufacturer's sheet. Read that, not a rewritten specification.

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