anti-Synaptophysin mouse, monoclonal, SY38, 0.2 ml, plastic

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anti-Synaptophysin mouse, monoclonal, SY₃₈, 50 µg/ml, affinity purified (made by PROGEN), Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +4 °C

This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.

High epitope affinity
Highly specific detection of the target protein
Unconjugated
Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence

SY 38 represents an excellent marker for several neuroendocrine, neuronal and adrenal tumors. Neuronal and adrenal tumors such as pheochromocytomas, paragangliomas, neuroblastomas, ganglioneuroblastomas. Neuroendocrine tumors of epithelial origin: Pancreatic islet cell carcinoma, bronchial and gastrointestinal carcinoids, medullary carcinoma of thyroid. Polypeptide reacting: 38 kDa transmembrane glycoprotein of presynaptic vesicles. Tested cultured cell lines: rat PC-12 cell line.

Application

Immunocytochemistry (ICC): assay dependentImmunohistochemistry (IHC) - frozen: 1:50-1:200 (0.25-1 μg/ml, no protease treatment)Immunohistochemistry (IHC) - paraffin: 1:50-1:200 (0.25-1 μg/ml, microwave treatment recommended, no protease treatment)Western Blot (WB): 1:500-1:1,000 (0.05-0.1 μg/ml)

Mouse brain (courtesy of J. Heß, University Hospital Heidelberg)

Western blot analysis of mouse brain lysate with anti-Synaptophysin antibody. Western blot analysis was performed on 20 μg mouse brain lysate. The PVDF membrane was blocked with 5% dry milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-Synaptophysin mouse monoclonal, SY38 (Cat. No. 690012) was diluted in blocking buffer (antibody concentration 0.05 μg/ml) and incubated for 1 h at RT. The secondary antibody anti-mouse, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.

Western blot analysis of rat Ins-1 lysate with anti-Synaptophysin antibody. Western blot analysis was performed on 20 μg rat Ins-1 lysate. The PVDF membrane was blocked with 5% dry milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-Synaptophysin mouse monoclonal, SY38 (Cat. No. 690012) was diluted in blocking buffer (antibody concentration 0.1 μg/ml) and incubated for 1 h at RT. The secondary antibody anti-mouse, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.

Not a medical device / Not an IVD product

Technical Information

Application	ICC/IF IHC WB
Host species	mouse
Clonality	monoclonal
Reactivity antibody	rat cattle
Purification	Affinity chromatography
Conjugate	uncojugated
Concentration	50 µg/ml
Isotype	IgG1
Uniprot ID AK	P20488 (bovine), P08247 (human), Q62277 (mouse), P07825 (rat)
Clone	SY38
Target	Synaptophysin

Technical Documentation (PDF)

anti-Synaptophysin mouse, monoclonal, SY38, 0.2 ml, plastic

Description