Description
ROTI®Free Stripping Buffer, ready-to-use, Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +15 to +25 °C
The buffer offers a simple tool for removing antibodies and chemoluminescence staining from Western blot membranes, allowing the valuable membranes to be analysed several times. Excellent when several proteins are to be detected in the same extract, or e.g. when in addition to a specific analysis, proteins in the respective lanes have to be semi-quantified (Kaufmann et. al. (1987) Anal. Biochem. 161:89-95). Suitable for nitrocellulose and PVDF-membranes.
- Efficient
- Well-tried formulation
- With β-mercaptoethanol
- For stripping under heating
- Low-priced
Wash the membrane in TBST-buffer (Art. No. 1061.1), incubate in ROTI®Free Stripping-buffer in closed hood for 30 min at 56 °C (e. g. in shaking water bath). To strip AP-conjugates, we recommend incubation at 70 °C for 30-60 min. After washing the membrane twice in TBST for 20 min each time, it should first be blocked, before the first antibody is added. The stripping process can be checked by brief chemiluminescence staining after washing in TBST. A slight reduction of the membrane-bound proteins and increase of the background may appear after repeated stripping. Store membranes after stripping wetpacked at +2 to +8 °C.
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Protein biology & Western blot
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Questions about ROTI®Free Stripping Buffer, ready-to-use, 250 ml, glass
What is this article?
ROTI®Free Stripping Buffer, ready-to-use, 250 ml, glass is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Protein extraction, purification & markers, inside Protein biology & Western blot, in Life Science & Microbiology.
Extraction reagents break the cell and keep the protein soluble. Purification resins and columns, often an affinity tag such as His, pull one protein out. A marker is a set of known masses run in the next lane. The mass range of the marker has to cover the protein being sought.
Protein work separates a mixture, then asks which protein is which. Extraction and purification take the protein out of the cell. A marker shows the masses. A Western blot moves the separated proteins onto a membrane and probes them with an antibody. The membrane, nitrocellulose or PVDF, has to bind the protein and survive the washes. Antibodies and methods are in PubMed. Reference material is at ATCC.
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The category is Protein biology & Western blot. The sub-category is Protein extraction, purification & markers. The catalogue is Life Science & Microbiology.
Shelves beside this one are Blotting membranes & papers and Protein biology (other).
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