Description
ROTIPHORESE® 50x TAE Buffer is a 50x concentrated buffer concentrate supplied for nucleic acid electrophoresis. The product is Density is specified as 1,086 g/cm³. Molecular biology laboratories rely on TAE buffer systems to maintain ionic strength and pH during DNA and RNA separation, enabling reproducible fragment migration in horizontal gel electrophoresis. This concentrate from manufacturer Carl ROTH supports gel electrophoresis workflows and related gel buffer solution needs across life-science research and teaching laboratories. The 50x presentation reduces storage volume while allowing flexible preparation of running buffer and gel buffer according to experimental scale. Application areas include electrophoresis of nucleic acids where stable buffering capacity is essential for clear band resolution and consistent analytical results in routine and advanced molecular biology protocols used for sizing, purity checks, and preparative separations.
This article is part of Biological buffers (Tris, HEPES, MOPS), in Buffers & pH solutions.
Key features
- Pack sizes: 1 l, 5 l, 2.5 l
- Density: 1,086 g/cm³
- Storage temperature: +15 to +25 °C
- Concentration: 50x buffer concentrate
- Technical Information Sheet:
- Safety Data Sheet:
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Questions about ROTIPHORESE® 50x TAE Buffer, 50x conc. buffer concentrate, 1 l, glass
What is this article?
ROTIPHORESE® 50x TAE Buffer, 50x conc. buffer concentrate, 1 l, glass is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Biological buffers (Tris, HEPES, MOPS), inside Buffers & pH solutions, in Lab chemicals.
Tris, HEPES, MOPS and their relatives hold pH in a range that cells and proteins tolerate, with a pKa near the working pH. The useful range is roughly one unit either side of the pKa. A phosphate buffer and Tris are not interchangeable in a method that names one of them, because the ion itself has effects.
A buffer resists a change in pH when a little acid or base is added. It does that because a weak acid and its conjugate base are both present. A pH buffer used to calibrate a meter has a stated value at a stated temperature. A biological buffer such as Tris or HEPES is chosen because it holds the pH of a living system and does not poison it. Conductivity standards are the sister articles for a conductivity meter. The ions are on PubChem. Electrode calibration practice sits with EURAMET and BELAC.
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The category is Buffers & pH solutions. The sub-category is Biological buffers (Tris, HEPES, MOPS). The catalogue is Lab chemicals.
Shelves beside this one are pH buffers & conductivity standards and Buffers (other).
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