Description
EC 250/3 NUCLEODUR C18 Gravity, 5 µm is an analytical HPLC separation column for reversed-phase liquid chromatography in research, development, and quality-control laboratories. The column has a length of 250 mm and an internal diameter of 3 mm and is packed with 5 µm NUCLEODUR C18 Gravity particles that provide a hydrophobic C18 bonded phase for separating non-polar to moderately polar organic compounds. This EC-format HPLC column from manufacturer Macherey-Nagel is supplied as one piece per pack and is intended for use on standard analytical HPLC systems. The Gravity C18 chemistry supports routine quantitative assays, impurity profiling, and method development where stable retention and consistent peak shape are required. Analysts can operate the column with common aqueous–organic mobile phases based on water, acetonitrile, or methanol and suitable buffers according to established methods. The 250 mm bed length delivers useful resolving power for complex mixtures, while the 3 mm internal diameter helps limit solvent consumption relative to wider analytical formats. The single-column pack size simplifies installation, spare holding, and replacement in pharmaceutical, chemical, and life-science chromatography workflows that rely on reliable reversed-phase HPLC performance.
This article is part of HPLC columns, in HPLC columns & accessories. See also HPLC guard columns.
Key features
- Pack size: 1 pc
- Column type: EC HPLC separation column
- Stationary phase: NUCLEODUR C18 Gravity
- Particle size: 5 µm
- Column length: 250 mm
- EAN: 4046681065026
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Questions about EC 250/3 NUCLEODUR C18 Gravity, 5 µm
What is this article?
EC 250/3 NUCLEODUR C18 Gravity, 5 µm is the article on this page. Luminix Health distributes Macherey-Nagel for laboratory use. It sits in HPLC columns, inside HPLC columns & accessories, in Chromatography equipment.
An HPLC column is specified by the stationary phase, the particle size, the length and the internal diameter. A C18, 5 µm, 150 × 4.6 mm column is a different article from a C18, 3 µm, 100 × 2 mm column. The smaller bore belongs on a lower flow. A column has a direction and a pH range.
An HPLC column is a tube packed, or coated, with a stationary phase. The sample is carried through it by a liquid, and the compounds leave at different times. The phase, the particle size, the length and the internal diameter decide the separation. A guard column is a short sacrificial piece in front of the analytical column. Fittings and tubing have a bore that either preserves the peak or smears it. Compound names follow IUPAC. Data are on PubChem. Method grades often cite the European Pharmacopoeia or USP.
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