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The HPLC columns shelf sits in HPLC columns & accessories. Luminix Health supplies this range from a catalogue of 150.000 laboratory articles for hospitals, universities, pharma and industrial laboratories.
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HPLC column specifications: what to compare
Use this overview to compare HPLC columns on the seven specifications that shape your separation, from particle size to endcapping.
| Specification | What it means | Values in our range | Effect on the separation |
|---|---|---|---|
| Particle size (µm) | The diameter of the packing particles inside the column. | 5, 3 and 1.8 µm fully porous; 2.7 µm core-shell; 7–10 µm for preparative work. Examples: C18 column, 3 µm, 150 × 4.6 mm, C18 column, 1.8 µm, 50 × 2 mm. | Smaller particles give sharper peaks and faster runs, at higher back pressure. 1.8 µm columns call for a UHPLC system rated for that pressure. |
| Particle type | Fully porous particles, or core-shell particles: a solid core with a thin porous outer layer. | Both fully porous and core-shell columns. Example: core-shell C18 column, 2.7 µm, 100 × 3 mm. | Core-shell particles deliver efficiency close to sub-2 µm particles at a lower back pressure, so standard HPLC systems can run faster methods. |
| Pore size (Å) | The average diameter of the pores in the particle, where most of the interaction with the analytes takes place. | About 100–120 Å, 90 Å for core-shell and 300 Å wide-pore. Example: wide-pore (300 Å) C18 column, 5 µm, 150 × 4.6 mm. | Around 100 Å suits small molecules. Peptides and proteins need 300 Å pores to reach the bonded surface and give well-shaped peaks. |
| Length (mm) | The length of the packed bed, from inlet to outlet. | 30 to 250 mm, most often 50, 100, 125, 150 or 250 mm. Example: C18 column, 5 µm, 250 × 4.6 mm. | A longer column gives more resolution for complex samples; a shorter column shortens run time. Changing length? See method transfer. |
| Inner diameter (mm) | The internal diameter of the column tube. | Analytical: 2, 3, 4 and 4.6 mm. Preparative: 8 to 50 mm. Example: preparative C18 column, 5 µm, 250 × 21 mm. | A narrower column runs at a lower flow rate and uses less solvent, with higher sensitivity in LC-MS. Wider columns suit purification. |
| Stationary phase | The bonded phase on the particle surface that interacts with your analytes. | C18 (reversed-phase standard), C8, C4 (300 Å), phenyl-hexyl, biphenyl, PFP, polar-embedded RP, HILIC, CN and NH2. Examples: C8 column, 5 µm, 150 × 4.6 mm, HILIC column, core-shell 2.7 µm, 50 × 3 mm. | Sets selectivity. C8 retains less; phenyl phases add π-π interactions for aromatics; PFP separates halogenated and positional isomers; HILIC retains very polar compounds. |
| Endcapping | A post-treatment that caps residual silanol groups on the silica surface after bonding. | Most reversed-phase columns are endcapped ("ec" in the name); HILIC, silica, CN and NH2 phases are not. Example: endcapped C18 column, 5 µm, 150 × 4.6 mm. | Better peak shape for basic compounds and less tailing, which makes integration more reliable. |
Extend the life of your analytical column with a guard column of the same phase: see our HPLC guard columns.
The values above are typical and intended as general guidance. The binding specifications, including pH range, maximum pressure and temperature, and pore size, are on each product page and in the column documentation. Check that a column suits your method and the pressure limit of your system. Comparing options for several instruments or sites? Our specialists help you choose: request a quote or write to sales@luminixhealth.com.
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Questions about HPLC columns
Which particle size should I choose: 5, 3, 2.7 or 1.8 µm?
Start from your system. 5 µm and 3 µm columns run on any standard HPLC system: 5 µm is the robust choice for routine methods, 3 µm gives sharper peaks in the same run time. 1.8 µm columns give the highest efficiency and the shortest runs, but need a UHPLC system rated for the higher back pressure. Core-shell 2.7 µm is the middle way, with efficiency close to sub-2 µm particles at a lower pressure. Examples: C18 column, 3 µm, C18 column, 1.8 µm and core-shell C18 column, 2.7 µm. Always check the pressure limit on the product page.
C18, C8, phenyl-hexyl, PFP or HILIC: which phase suits which compounds?
C18 is the reversed-phase standard and the right first choice for most methods. C8 retains less and gives faster runs for hydrophobic compounds. Phenyl-hexyl adds π-π interactions, which helps with aromatics. PFP gives different selectivity for halogenated compounds, positional isomers and polar aromatics. HILIC retains very polar compounds that elute too early on C18. Examples: C8 column, phenyl-hexyl column, PFP column and HILIC column. Not sure which phase fits your sample? Send us your method details at sales@luminixhealth.com.
Which pore size do I need for peptides and proteins?
Pore size decides whether large molecules can reach the bonded phase. Columns with pores of about 100 Å are designed for small molecules. Peptides and proteins are too large for these pores and give broad peaks or poor recovery. For them, choose a wide-pore column with 300 Å pores: C4 with 300 Å pores for proteins, or C18 with 300 Å pores for peptides and smaller proteins. Example: wide-pore (300 Å) C18 column. The exact pore size of each column is on its product page.
How do I transfer a method to a different column length or inner diameter?
Keep the stationary phase and particle type the same, so selectivity stays comparable. When you change length or inner diameter, scale the flow rate and injection volume to the new column dimensions; a shorter column or smaller particles also let you shorten the gradient. Our HPLC method transfer page explains the approach, and our calculators help with the conversion. Protect the new column with a guard column of the same phase from our HPLC guard columns. For larger transfer projects, our specialists are glad to help at sales@luminixhealth.com.
Do I need a guard column?
A guard column is a short column with the same phase, placed before the analytical column. It traps particles and strongly bound matrix compounds before they reach the expensive column, so peak shape and pressure stay stable for longer. It pays off especially with matrix-rich samples, such as food, soil or biological extracts, and with methods that run many injections. Replace the guard cartridge when pressure rises or peaks broaden. Choose a guard with the same phase as your column; options are in HPLC guard columns. Unsure which guard fits your column? Ask sales@luminixhealth.com.
Why HPLC columns?
An HPLC column is specified by the stationary phase, the particle size, the length and the internal diameter. A C18, 5 µm, 150 × 4.6 mm column is a different article from a C18, 3 µm, 100 × 2 mm column. The smaller bore belongs on a lower flow. A column has a direction and a pH range.
An HPLC column is a tube packed, or coated, with a stationary phase. The sample is carried through it by a liquid, and the compounds leave at different times. The phase, the particle size, the length and the internal diameter decide the separation. A guard column is a short sacrificial piece in front of the analytical column. Fittings and tubing have a bore that either preserves the peak or smears it. Compound names follow IUPAC. Data are on PubChem. Method grades often cite the European Pharmacopoeia or USP.
This shelf belongs to HPLC columns & accessories, inside Chromatography equipment. Luminix Health distributes this range for laboratory use.
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The neighbouring shelves are HPLC fittings, tubing & inline filters, HPLC guard columns and HPLC accessories. The category above this shelf is HPLC columns & accessories.
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