Description
EC 250/4 NUCLEODUR HILIC, 5 µm is an EC HPLC separation column built for hydrophilic interaction chromatography of polar analytes. The column provides a 250 mm length, a 4 mm internal diameter, and a 5 µm NUCLEODUR HILIC stationary phase, corresponding to a widely used analytical format for robust HILIC separations. It is supplied as a single piece and matches standard EC HPLC hardware for straightforward system connection. NUCLEODUR HILIC chemistry targets hydrophilic compounds that are insufficiently retained under reversed-phase conditions, enabling complementary selectivity in method development and quality-control workflows. With 5 µm particles, the packing supports efficient peaks at pressures compatible with conventional HPLC pumps, while the 250 mm × 4 mm geometry offers good sample capacity and familiar method-transfer behaviour. This configuration is suitable for pharmaceutical, biochemical, and general analytical laboratories that require dedicated HILIC columns for polar metabolite, intermediate, or impurity work. The EC 250/4 NUCLEODUR HILIC column from manufacturer Macherey-Nagel arrives as one unit per pack for direct installation and stock management.
This article is part of HPLC columns, in HPLC columns & accessories. See also HPLC guard columns.
Key features
- Pack size: 1 pc
- Column type: EC HPLC separation column
- Stationary phase: NUCLEODUR HILIC
- Particle size: 5 µm
- Column length: 250 mm
- EAN: 4046681061240
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Questions about EC 250/4 NUCLEODUR HILIC, 5 µm
What is this article?
EC 250/4 NUCLEODUR HILIC, 5 µm is the article on this page. Luminix Health distributes Macherey-Nagel for laboratory use. It sits in HPLC columns, inside HPLC columns & accessories, in Chromatography equipment.
An HPLC column is specified by the stationary phase, the particle size, the length and the internal diameter. A C18, 5 µm, 150 × 4.6 mm column is a different article from a C18, 3 µm, 100 × 2 mm column. The smaller bore belongs on a lower flow. A column has a direction and a pH range.
An HPLC column is a tube packed, or coated, with a stationary phase. The sample is carried through it by a liquid, and the compounds leave at different times. The phase, the particle size, the length and the internal diameter decide the separation. A guard column is a short sacrificial piece in front of the analytical column. Fittings and tubing have a bore that either preserves the peak or smears it. Compound names follow IUPAC. Data are on PubChem. Method grades often cite the European Pharmacopoeia or USP.
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