Description
EC 250/4 NUCLEOSIL 500-5 C18 PPN is an EC HPLC separation column for reversed-phase chromatographic applications. It features a bed length of 250 mm and an internal diameter of 4 mm, packed with NUCLEOSIL 500-5 C18 PPN, a C18-modified silica phase selected for analytical separations that benefit from this pore and particle configuration. The column is delivered as one piece per pack and is intended for use on standard HPLC instruments in research, quality control and method-transfer environments. The EC column body provides conventional end-fitting geometry for straightforward installation in analytical LC systems running isocratic or gradient methods. From manufacturer Macherey-Nagel, this NUCLEOSIL 500-5 C18 PPN column combines the familiar 250 mm × 4 mm analytical format with C18 selectivity for small-molecule and related compound separations. Net weight is 0,24 kg. Operate within the pressure, solvent and equilibration guidelines customary for 5 µm silica-based C18 HPLC columns to maintain performance and column lifetime.
This article is part of HPLC columns, in HPLC columns & accessories. See also HPLC guard columns.
Key features
- Pack size: 1 pc
- Column length: 250 mm
- Internal diameter: 4 mm
- Stationary phase: NUCLEOSIL 500-5 C18 PPN
- Column format: EC HPLC separation column
- EAN: 4046681081910
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Questions about EC 250/4 NUCLEOSIL 500-5 C18 PPN
What is this article?
EC 250/4 NUCLEOSIL 500-5 C18 PPN is the article on this page. Luminix Health distributes Macherey-Nagel for laboratory use. It sits in HPLC columns, inside HPLC columns & accessories, in Chromatography equipment.
An HPLC column is specified by the stationary phase, the particle size, the length and the internal diameter. A C18, 5 µm, 150 × 4.6 mm column is a different article from a C18, 3 µm, 100 × 2 mm column. The smaller bore belongs on a lower flow. A column has a direction and a pH range.
An HPLC column is a tube packed, or coated, with a stationary phase. The sample is carried through it by a liquid, and the compounds leave at different times. The phase, the particle size, the length and the internal diameter decide the separation. A guard column is a short sacrificial piece in front of the analytical column. Fittings and tubing have a bore that either preserves the peak or smears it. Compound names follow IUPAC. Data are on PubChem. Method grades often cite the European Pharmacopoeia or USP.
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