Description
EC 250/4.6 NUCLEODUR 100-3 is an EC HPLC separation column built for analytical liquid chromatography applications that benefit from extended bed length. The column measures 250 mm in length with an internal diameter of 4,6 mm, offering additional theoretical plates compared with shorter formats while remaining compatible with conventional HPLC systems. It carries the NUCLEODUR 100-3 designation and is delivered as one piece for direct instrument mounting. Laboratories employ 250 mm × 4,6 mm EC columns when higher resolution or longer retention windows are required for complex sample matrices in research, quality control and routine analysis. Stable EC end-fittings support reliable connections under typical analytical pressures during isocratic and gradient elution. This column is produced by Macherey-Nagel and suits pharmaceutical, environmental, food and industrial chemistry workflows that standardise on 4,6 mm ID hardware. Supplied as a single unit, the EC 250/4.6 NUCLEODUR 100-3 column simplifies method transfer between instruments that already use the same length and diameter. Clear dimensional labelling aids inventory management and helps analysts select the appropriate geometry for demanding separations without altering established system plumbing.
This article is part of HPLC columns, in HPLC columns & accessories. See also HPLC guard columns.
Key features
- Pack size: 1 pc
- Product type: EC HPLC separation column
- Stationary phase designation: NUCLEODUR 100-3
- Column length: 250 mm
- Internal diameter: 4,6 mm
- EAN: 4046681064708
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Questions about EC 250/4.6 NUCLEODUR 100-3
What is this article?
EC 250/4.6 NUCLEODUR 100-3 is the article on this page. Luminix Health distributes Macherey-Nagel for laboratory use. It sits in HPLC columns, inside HPLC columns & accessories, in Chromatography equipment.
An HPLC column is specified by the stationary phase, the particle size, the length and the internal diameter. A C18, 5 µm, 150 × 4.6 mm column is a different article from a C18, 3 µm, 100 × 2.1 mm column. The smaller bore belongs on a lower flow. A column has a direction and a pH range.
An HPLC column is a tube packed, or coated, with a stationary phase. The sample is carried through it by a liquid, and the compounds leave at different times. The phase, the particle size, the length and the internal diameter decide the separation. A guard column is a short sacrificial piece in front of the analytical column. Fittings and tubing have a bore that either preserves the peak or smears it. Compound names follow IUPAC. Data are on PubChem. Method grades often cite the European Pharmacopoeia or USP.
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The category is HPLC columns & accessories. The sub-category is HPLC columns. The catalogue is Chromatography equipment.
Shelves beside this one are HPLC fittings, tubing & inline filters, HPLC guard columns and HPLC accessories.
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Luminix Health distributes this article from the heart of Europe. Articles in stock ship within 2–5 business days. Standard delivery is free from €250 excl. VAT. Cold-chain goods and dangerous goods under ADR are never included in that free delivery. The terms are in the Shipping Policy. The full brand index is on shop by brand. Who we are is on about us.
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The seven catalogues are Labware, Lab chemicals, Lab instruments, Lab consumables, Chromatography equipment, Lab safety equipment and Life Science & Microbiology.

