Description
EC 50/4.6 NUCLEODUR HILIC, 5 µm is an EC HPLC separation column for hydrophilic interaction liquid chromatography. The column length is 50 mm with an internal diameter of 4,6 mm, and the bed is packed with 5 µm NUCLEODUR HILIC particles. It is offered as a single-piece pack for analytical HPLC applications focused on polar and hydrophilic analytes that are poorly retained under conventional reversed-phase conditions. HILIC operation with aqueous–organic mobile phases containing a high organic fraction supports selective separation of polar pharmaceuticals, metabolites and other hydrophilic species. The compact 50 mm length helps keep analysis times short during method development and routine work, while the 4,6 mm internal diameter matches a classic analytical column format familiar across many HPLC laboratories. The 5 µm particle size provides a practical balance of efficiency and back-pressure on standard liquid chromatography systems. This EC-format NUCLEODUR HILIC column from manufacturer Macherey-Nagel sits within the HPLC chromatography column category and is supplied ready for use in research and quality-control instruments performing polar-compound separations.
This article is part of HPLC columns, in HPLC columns & accessories. See also HPLC guard columns.
Key features
- Pack size: 1 pc
- Product type: EC HPLC separation column
- Stationary phase: NUCLEODUR HILIC
- Particle size: 5 µm
- Column length: 50 mm
- EAN: 4046681061158
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Questions about EC 50/4.6 NUCLEODUR HILIC, 5 µm
What is this article?
EC 50/4.6 NUCLEODUR HILIC, 5 µm is the article on this page. Luminix Health distributes Macherey-Nagel for laboratory use. It sits in HPLC columns, inside HPLC columns & accessories, in Chromatography equipment.
An HPLC column is specified by the stationary phase, the particle size, the length and the internal diameter. A C18, 5 µm, 150 × 4.6 mm column is a different article from a C18, 3 µm, 100 × 2.1 mm column. The smaller bore belongs on a lower flow. A column has a direction and a pH range.
An HPLC column is a tube packed, or coated, with a stationary phase. The sample is carried through it by a liquid, and the compounds leave at different times. The phase, the particle size, the length and the internal diameter decide the separation. A guard column is a short sacrificial piece in front of the analytical column. Fittings and tubing have a bore that either preserves the peak or smears it. Compound names follow IUPAC. Data are on PubChem. Method grades often cite the European Pharmacopoeia or USP.
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