Bromelain, 1200 GDU/g, 10 g, plastic

Highly active thiol proteinase (non-specific cystein protease), glycoprotein. Enzyme from Bromeliaceae, used in biochemistry and for meat tenderising. Preferable digestion at lysin, alanin, tyrosin and glycin. Degrades hemoglobin, gluten, collagen, globulin, casein and gelatin at pH 4-9. Cream-coloured, hygroscopic powder.Bromelain is activated by cystein, bisufite salts, NaCN, Na₂S, H₂S and benzoate. The definite pH optimum depends on substrate and reaction parameters. pH stability of bromelain increases drastically after binding to the substrate.

One GDU (gelatin digestion unit) will hydrolyse 1.0 mg of amino nitrogen from gelatin in 20 minutes at pH 4.5 at 45 °C. One bromelain tyrosine unit (BTU) corresponds to 1.1 GDU. 1200 GDU/g correspond to approx. 2.4 FIP-units/mg.

Working concentration: substrate dependent.pH working range: 4.0-8.0, pH optimum: 5-6, stabile at pH 3.0-6.5Temperature optimum: 50-65 °CInhibitors: Hg²⁺, Ag⁺, Cu²⁺, antitrypsin, estatin, iodacetate, TLCK, TPCK, and temperatures of over 70 °C