Description
Papain, min. 30 000 USP-U/mg, for biochemistry, Storage temp. +4 °C, CAS No. 9001-73-4, EC No. 232-627-2
A non-specific endopeptidase from Papaya latex. Has a very wide substrate range and cleaves proteins more efficiently than trypsin, for example. Is capable of not only hydrolysing peptide bonds, but also acts effectively as an esterase and thiol-esterase.
30.000 USP (Casein) Units is approx. 2,5 FIP-Units (BAEE-Units). One USP Unit of Papain activity releases the equivalent of 1 µg tyrosine form a specified casein substrate under assay conditions specified by the USP.
Working concentration: Dilution immediately before application in a buffer with approx. 5 mM L-CysteinepH optimum: 6.0Temperature optimum: 65 °CActivation and stabilization: EDTA, Cysteine and Dimercaptopropanol. DTT may be added in orderto increase activity.
| Additional info | Source: Carica papaya |