500 bp-DNA-ladder

500 bp-DNA-ladder is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Protein extraction, purification & markers, inside Protein biology & Western blot, in Microbiologie.

Extraction reagents break the cell and keep the protein soluble. Purification resins and columns, often an affinity tag such as His, pull one protein out. A marker is a set of known masses run in the next lane. The mass range of the marker has to cover the protein being sought.

Protein work separates a mixture, then asks which protein is which. Extraction and purification take the protein out of the cell. A marker shows the masses. A Western blot moves the separated proteins onto a membrane and probes them with an antibody. The membrane, nitrocellulose or PVDF, has to bind the protein and survive the washes. Antibodies and methods are in PubMed. Reference material is at ATCC.

The category is Protein biology & Western blot. The sub-category is Protein extraction, purification & markers. The catalogue is Microbiologie.

Shelves beside this one are Blotting membranes & papers and Protein biology (other).

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The seven catalogues are Labware, Lab chemicals, Lab instruments, Lab consumables, Chromatography equipment, Lab safety equipment and Life Science & Microbiology.