HPLC & LC-MS grade solvents

HPLC grade is made for UV and other optical detectors: the key properties are UV transmission, low residue and few particles. Gradient grades add a flat baseline over the whole gradient, as with acetonitrile, HPLC gradient grade, 1 l, glass. LC-MS grade is also tested for what disturbs a mass spectrometer, such as metal ions, adduct-forming impurities and background ions, for example methanol, LC-MS grade, 1 l, glass. Use LC-MS grade whenever an MS detector is in the system. Questions on a method: sales@luminixhealth.com.

Organic solvents such as acetonitrile and methanol are supplied in glass, which does not release plasticisers or other extractables into the solvent. For LC-MS water, plastic can be the better choice, because glass can release sodium and other ions that show up as adducts in the mass spectrum. An example is water, LC-MS grade, basic, 1 l, plastic. Whatever the bottle, rinse your solvent reservoir with the same solvent and avoid plastic squeeze bottles for topping up. More waters are in this collection.

Yes. A solvent inlet filter on the end of the suction line keeps particles out of the pump, check valves and column, and it also weighs the line down so it stays in the solvent. Replace it when flow drops or the pump struggles to prime, and never let it run dry. Examples are the HPLC solvent inlet filter, Ø 28 mm and the HPLC solvent inlet filter, Ø 23 mm; check that the diameter fits the neck of your reservoir. Columns and spare parts: HPLC columns and accessories.

Dissolved air forms bubbles in the pump and detector, which gives pressure ripple and baseline noise. Most systems have an inline degasser; otherwise degas the eluent briefly in an ultrasonic bath, under vacuum or by helium sparging. Keep the reservoir closed with a cap that only lets the line through, so the eluent does not take up air, dust or evaporate. Make aqueous buffers fresh, because they grow microbes within days. Suitable baths are in ultrasonic baths and cleaners. Questions: sales@luminixhealth.com.

HPLC grade limits the absorbance and the residue that would appear as peaks or baseline drift. LC-MS grade goes further on metal ions and on particles that would suppress a mass spectrum. A solvent that is only analytical grade is a false economy on an LC-MS system.

A solvent dissolves. The grade says which impurities are low enough for the method. Analytical grade is the ordinary pure solvent. HPLC and LC-MS grades limit the absorbance and the ions that would become peaks or background. A dry solvent has had water removed. A deuterated solvent is for NMR, where the hydrogen signal would otherwise drown the sample. Technical grade is for cleaning and for synthesis that does not need a chromatogram. Identity is on PubChem. Names follow IUPAC. Hazards follow ECHA, the CLP Regulation and REACH.

This shelf belongs to Solvents, inside Lab chemicals. Luminix Health distributes this range for laboratory use.

The neighbouring shelves are Analytical grade solvents (p.a.), Dry & deuterated solvents (NMR, synthesis), Technical & synthesis grade solvents and Green & alternative solvents. The category above this shelf is Solvents.

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