TLC & HPTLC plates

HPTLC plates have a finer and more even layer than standard TLC plates. That gives sharper spots, shorter run times and better quantification, especially when you apply samples and scan plates with instruments. Standard TLC plates remain the practical choice for quick reaction monitoring and screening. Choose by whether you need a yes/no answer or a number. Both are available on glass or foil. Compare plates in TLC and HPTLC plates. Need advice for your set-up? Mail sales@luminixhealth.com.

Glass backing is rigid, heat resistant and chemically inert, and the plate is easy to document and archive. Aluminium and plastic backings can be cut to size with scissors, are light and cost less. Avoid aluminium with strongly acidic or alkaline reagents, which attack the metal. Choose by how you develop and stain. Chambers and tools are in TLC chambers and accessories. For a quote or a second opinion, write to sales@luminixhealth.com.

F254 means the layer contains a fluorescent indicator that glows green under UV light at 254 nm. Compounds that absorb UV at that wavelength quench the glow and appear as dark spots, so you can see them without staining. On plates without an indicator, you make spots visible with a spray or dip reagent instead. Lamps are in cold light sources and lighting. Questions about a specific product? Contact sales@luminixhealth.com.

On plates with an indicator, use a UV lamp first. Otherwise, or in addition, apply a spray or dip reagent that reacts with your compounds, such as ninhydrin for amino acids or bromocresol green for acids, and heat the plate if the method asks for it. A sprayer or dipping chamber gives an even coating. Spray and dip reagents are often corrosive or toxic, so apply them in a fume cupboard. Reagents are in dyes, stains and indicators. Unsure which one fits? Ask sales@luminixhealth.com.

A TLC plate is a backing, glass, aluminium or plastic, coated with silica or alumina of a stated thickness. HPTLC uses a finer particle and a thinner layer. Indicator plates fluoresce so a dark spot shows under UV. Plate size and layer thickness are the specification.

Thin-layer chromatography runs a liquid up a plate coated with a sorbent. The compounds move different distances and are seen as spots. HPTLC is the same method with a finer layer. The plate, the solvent mixture and the chamber saturation decide the result. It is the quick look before, or beside, a column method. The substances are on PubChem, named by IUPAC.

This shelf belongs to TLC, inside Chromatography equipment. Luminix Health distributes this range for laboratory use.

The neighbouring shelves are TLC chambers & accessories. The category above this shelf is TLC.

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