Light & fluorescence microscopes

An upright microscope looks at a slide from above and is the standard for stained sections, smears and routine biology. An inverted microscope has the objectives under the stage, so you can look at cells through the bottom of a flask, dish or well plate without opening it. Examples: the inverted microscope with 5 W LED and trinocular head and the inverted microscope with phase contrast, 4–40X. Compare at sales@luminixhealth.com.

You need fluorescence when you work with stained or labelled targets, such as DAPI-stained nuclei, GFP-expressing cells or immunofluorescence. Epi-fluorescence lights the sample through the objective with a specific wavelength, and filters let only the emitted light through, so the target lights up against a dark background. Match the filter sets to your dyes. An example is the fluorescence microscope with 20 W halogen transmitted light and 100 W epi-fluorescence, with blue and green filters. Fluorescence kits for existing microscopes only fit specific series, so ask sales@luminixhealth.com first.

Eyepieces give the sharpest live view and suit routine looking and teaching. A camera or screen head lets you save images, measure and share results, which helps with documentation, training and discussing findings with colleagues. You can add a camera to a trinocular microscope later, or choose a model with a built-in camera. An example is the biological microscope with digital head, integrated 3 MP camera and USB 2.0, objectives up to 100X oil. For high-magnification work, check the camera resolution against what you need to see. Browse light and fluorescence microscopes.

Total magnification is the objective times the eyepiece, usually 10×. Around 40× to 100× shows tissue structure and larger organisms, 400× shows most cells, and 1000× with oil immersion is needed for bacteria and fine cell detail. More magnification only helps if the optics resolve it, so look at objective quality and not just the number. An example covering 100× to 1000× is the laboratory microscope for activated sludge analysis, with phase contrast, bright-field and dark-field. For stereo work at low magnification, see stereo microscopes.

A light microscope looks through a thin specimen. A fluorescence microscope adds a light source and filters matched to a dye, so only the labelled structure shows. The filter set has to match the dye's excitation and emission. A bright-field microscope without those filters cannot do that experiment.

A light microscope forms an image with glass lenses and transmitted or reflected light. A stereo microscope is the lower-magnification instrument for dissecting and for looking at a surface. Fluorescence needs a light source and filters matched to the dye. Histology is the preparation that makes a tissue readable: fixation, a cassette, a section, a stain. The objective's magnification and numerical aperture decide the resolution. The slide and the cover glass decide whether the section sits in the focal plane. Stains and dyes as substances are identified on PubChem. Papers on the methods are on PubMed.

This shelf belongs to Microscopy & histology, inside Lab instruments. Luminix Health distributes this range for laboratory use.

The neighbouring shelves are Microscope slides & cover glasses, Microscope objectives, eyepieces & optics, Histology cassettes & moulds, Counting chambers & haemocytometers, Stereo microscopes, Histology reagents & consumables, Microscopy accessories, Cold light sources & lighting and Digital & USB microscopes. The category above this shelf is Microscopy & histology.

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