Microcentrifuge & reaction tubes

The size follows the instrument and the sample volume. Thin-walled 0.2 ml tubes fit standard PCR cyclers, for example the PCR tube, 0.2 ml; 0.5 ml tubes, such as the PCR tube, 0.5 ml, suit older cyclers and small reaction volumes. The 1.5 ml tube is the everyday standard for preparations, digests and storage, like the sterile safety-cap tube, 1.5 ml. Choose 2 ml for larger volumes or when you need room to mix, for example the sterile safety-cap tube, 2.0 ml. Check that your rotor and racks fit.

A snap cap opens and closes with one hand, which is fast for routine work at the bench. Safety caps lock so they do not pop open when heated or centrifuged, as with the sterile safety-cap tube, 1.5 ml. Choose a screw cap when samples are stored for a long time, frozen, shipped or boiled, because the thread and seal hold better and limit evaporation. The low-binding self-standing screw-cap microtube, 1.5 ml is an example. Screw caps also reduce the risk of aerosols when you open tubes with infectious material.

Every tube has a maximum centrifugal force, stated as × g on the product page; check it against your protocol and rotor. Values differ widely between tubes. The sterile safety-cap tube, 1.5 ml is rated up to 30,000 × g and the 2.0 ml version up to 20,000 × g. The black reaction tube, 1.5 ml, made for light-sensitive samples, goes up to 15,000 × g, and the graduated round-bottom tube, 2 ml up to 10,000 × g. Note that rpm is not the same as × g: convert using your rotor radius.

These answer different needs. Low-binding tubes have a surface that holds back less protein or nucleic acid, so you recover more of a small or dilute sample, for example the low-binding screw-cap microtube, 1.5 ml. Use them for enzymes, antibodies, low-concentration DNA and quantitative work. Sterile tubes are about contamination: they suit cell culture, microbiology and sensitive molecular work. The sterile safety-cap tube, 1.5 ml is also free from DNase, RNase and PCR inhibitors. For a precious sample in an aseptic workflow, you may need both. Questions: sales@luminixhealth.com.

A microcentrifuge tube, the familiar 1.5 mL or 2 mL flip-cap tube, holds a small reaction or a spin-down. The cap style, the centrifugal-force rating and whether the tube is low-binding are the specification. A PCR tube is thinner and is a different article.

A microplate is an array of wells so the same step can be done many times. A deep-well plate holds more volume. A PCR plate or a strip of tubes is thin-walled so the temperature of a cycler reaches the liquid quickly. A seal keeps the well from evaporating. The well number, the working volume and whether the plate is skirted for a robot are the choices. Diagnostic test systems are listed under ISO in vitro diagnostic systems. Methods are on PubMed.

This shelf belongs to Microplates, PCR & reaction tubes, inside Lab consumables. Luminix Health distributes this range for laboratory use.

The neighbouring shelves are PCR plates, tubes & strips, Microplates, Plate seals & sealing films and Deep-well plates. The category above this shelf is Microplates, PCR & reaction tubes.

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