Description
anti-Desmoglein 1/2 mouse , monoclonal, DG 3.10, 50 µg/ml, affinity purified (made by PROGEN), Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +4 °C
This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.
- High epitope affinity
- Highly specific detection of the target protein
- Unconjugated
- Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence
DG 3.10 recognizes desmoglein 1 + 2 (desmosome-specific cadherins) in desmosome-bearing cells; does not react with cells containing plaque-bearing junctions of non-desmosomal type. Tumors specifically detected: all epithelium-derived tumors, notably carcinomas, and meningiomas. Polypeptide reacting: Mr 165 000 Desmoglein, 'band 3' polypeptide of the desmosomal complex. Epitope recognized has been localized at the repeating unit domain ('RUD') of the cytoplasmic C-terminal part of the polypeptide. Reactivity on cultured cell lines: A-431, MDBK.
Immunocytochemistry (ICC): Assay dependentImmunohistochemistry (IHC) - frozen: 1:10-1:100 (0.5-5 μg/ml) Immunohistochemistry (IHC) - paraffin: 1:10-1:100 (0.5-5 μg/ml, microwave treatment recommended) Western Blot (WB): 1:2000-1:4000 (12,5 ng/ml - 25 ng/ml)
| Application | ICC/IF IHC WB |
| Host species | mouse |
| Clonality | monoclonal |
| Reactivity antibody | rat cattle |
| Purification | Affinity chromatography |
| Conjugate | uncojugated |
| Concentration | 50 µg/ml |
| Isotype | IgG1 |
| Uniprot ID AK | Q02413 (human), D3ZM39 (rat), Q03763 (bovine) |
| Clone | DG 3.10 |
| Target | 'band 3' polypeptide of isolated desmosomes from bovine muzzle epidermis |