Description
DAPI is a high-purity fluorescent dye supplied at min. 98 % for biochemistry applications. With CAS number 28718-90-3 and empirical formula C₁₆H₁₇Cl₂N₅, it has a molar mass of 350,3 g/mol and is widely used for nucleic-acid staining in fluorescence microscopy and related life-science workflows. The compound binds preferentially to AT-rich regions of double-stranded DNA, producing a strong blue fluorescence signal that supports nuclear counterstaining, chromosome visualisation and cell-cycle studies. Offered from manufacturer Carl ROTH for biochemistry, the material suits protocols in cell biology, histology and molecular imaging. Its defined purity grade supports reproducible staining results when prepared according to standard laboratory procedures. Researchers value DAPI for clear nuclear delineation in multicolour fluorescence experiments and for quantitative DNA-related assays. Handle and dissolve according to established biochemical practice to maintain dye integrity throughout sample preparation and imaging.
Key features
- Assay (HPLC): ≥98 %
- CAS number: 28718-90-3
- Pack sizes: 10 mg, 25 mg, 100 mg
- EG nr.: 249-186-7
- Purity: min. 98 %
- Molar mass: 350,3 g/mol
- Technical Information Sheet:
- Safety Data Sheet:
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Questions about DAPI, min. 98 %, for biochemistry, 10 mg, glass
What is this article?
DAPI, min. 98 %, for biochemistry, 10 mg, glass is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Fluorescent dyes & labels, inside Dyes, stains & indicators, in Lab chemicals.
A fluorescent dye absorbs at one wavelength and emits at a longer one. As a label it is attached to a protein, a nucleic acid or a cell. Excitation and emission maxima, and the reactive group if it must conjugate, are the specification. The colour name does not tell you the wavelengths.
A dye or a stain binds, or changes colour, so that something invisible becomes visible. An indicator changes at a pH or a redox point. A fluorescent dye absorbs one wavelength and emits another, which is how a microscope or a plate reader picks it out. The excitation and emission, or the transition interval, are the specification. The colour name on the label is not. Structures are on PubChem under IUPAC names. Methods are in the PubMed literature.
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The category is Dyes, stains & indicators. The sub-category is Fluorescent dyes & labels. The catalogue is Lab chemicals.
Shelves beside this one are Microscopy stains & staining solutions and Dyes & pigments.
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The seven catalogues are Labware, Lab chemicals, Lab instruments, Lab consumables, Chromatography equipment, Lab safety equipment and Life Science & Microbiology.