Description
ROTI®DAB Kit, for immunochemistry, Storage temp. +4 °C
DAB substrate kit for detection of peroxidase (HRP) activity on immunoblots, immunohisto- and cytochemical slides and during in situ hybridization.
ROTI®DAB Kit contains substrate, buffer solution and activation reagent, therefore including all three solutions necessary for HRP detection reaction. Application is very simple - all three kit solutions are diluted equally in water, and the blotting filter membrane, or the slides, are then incubated in this substrate solution. Since the solutions are delivered in drop dispensers, dosing is most convenient.
DAB is oxidized by HRP, forming a brown precipitate, which is insoluble in aqueous and organic solvents. This precipitate can be detected in visible light and does not bleach during long-term storage.
Addition of the DAB Metal Enhancer (Art. No. 9204) doubles the signal strength. Slides may be permanently mounted in hydrophilic, or, after dehydration, in hydrophobic mounting medium.
DAB solution (Art. No. 9869), DAB buffer (Art. No. 9870), and Activation reagent (Art. No. 9871) in dropper bottles. Contents of this Kit may not be bought separately.
| Application | Western, in situ, Histochemistry |
| Enzyme | Horseradish Peroxidase |
| Detection | Colour (brown) |
| Signal product | Precipitate (Blot) |
Compliance & Safety Information
DangerHazard Statements (H-Phrases)
Precautionary Statements (P-Phrases)
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Questions about ROTI®DAB Kit, for immunochemistry, 1 kit, cardboard
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ROTI®DAB Kit, for immunochemistry, 1 kit, cardboard is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Moisture analysers, inside Balances & scales, in Lab instruments.
A moisture analyser is a balance with a halogen or infrared heater. It records the loss of mass as a sample dries and reports that loss as moisture. The temperature program and the sample mass decide whether the number is water or is decomposition.
A balance compares a mass with a standard. An analytical balance reads to 0.1 mg or finer and needs a draft shield and a stable bench. A precision balance is coarser and faster. A platform scale takes a heavier load. The reading is only as good as the calibration chain back to the kilogram, which is why test weights and a certificate exist. A moisture analyser is a balance with a heater: it records the loss of mass as water leaves. The unit of mass is kept by the BIPM, compared across Europe by EURAMET, and accredited by BELAC. NIST publishes the same measurement foundations.
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