Description
ROTI®Free Stripping Buffer 2.0 is a ready-to-use stripping reagent for Western blot workflows, It is intended for removal of antibodies from blotted membranes so that membranes can be re-probed in sequential detection experiments. Physical data from the manufacturer indicate a density of approximately 1 g/cm³ and a boiling point around 100 °C, consistent with aqueous buffer handling on the bench. The formulation supports blotting laboratories that need a convenient, ready-to-use strip solution without additional preparation steps. Use it where controlled antibody removal and membrane re-use are part of multi-target immunodetection strategies. The glass packaging supports secure storage and dispensing during repeated stripping cycles in protein analysis labs.
This article is part of Protein extraction, purification & markers, in Protein biology & Western blot.
Key features
- Pack sizes: 250 ml, 1 l
- Density: ~1 g/cm³
- Boiling point: ~100 °C
- Product type: Stripping buffer
- Grade: Ready-to-use
- Technical Information Sheet:
- Safety Data Sheet:
Compliance & Safety Information
WarningHazard Statements (H-Phrases)
Precautionary Statements (P-Phrases)
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Questions about ROTI®Free Stripping Buffer 2.0, ready-to-use, 250 ml, glass
What is this article?
ROTI®Free Stripping Buffer 2.0, ready-to-use, 250 ml, glass is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Protein extraction, purification & markers, inside Protein biology & Western blot, in Microbiology lab supplies.
Extraction reagents break the cell and keep the protein soluble. Purification resins and columns, often an affinity tag such as His, pull one protein out. A marker is a set of known masses run in the next lane. The mass range of the marker has to cover the protein being sought.
Protein work separates a mixture, then asks which protein is which. Extraction and purification take the protein out of the cell. A marker shows the masses. A Western blot moves the separated proteins onto a membrane and probes them with an antibody. The membrane, nitrocellulose or PVDF, has to bind the protein and survive the washes. Antibodies and methods are in PubMed. Reference material is at ATCC.
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The category is Protein biology & Western blot. The sub-category is Protein extraction, purification & markers. The catalogue is Microbiology lab supplies.
Shelves beside this one are Blotting membranes & papers and Protein biology (other).
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The seven catalogues are Labware, Lab chemicals, Lab instruments, Lab consumables, Chromatography equipment, Lab safety equipment and Life Science & Microbiology.
