Description
ROTI®Lumin is a fluorescence-free, 10× concentrated substrate kit supplied as 2 × 120 ml for protein detection and cytoimmunochemistry applications. Designed for life-science and biochemistry laboratories, it supports luminescence-based readout where low background fluorescence is important for clear signal interpretation. The concentrated format allows flexible working dilutions matched to blot, membrane or cellular assay protocols used in routine immunodetection. Packed as a ready kit, the dual 120 ml presentation suits shared bench stocks and repeated experimental series. ROTI®Lumin is offered by manufacturer Carl ROTH within its range of biochemical substrates, giving researchers a practical reagent option for protein visualisation workflows. Users integrate the concentrate into established cytoimmunochemistry and related detection schemes, adjusting dilution and incubation to their antibody systems and imaging setup. The fluorescence-free specification helps maintain clean baselines when sensitive cameras or scanners record chemiluminescent or related signals from labelled targets.
This article is part of Protein extraction, purification & markers, in Protein biology & Western blot.
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- Technical Information Sheet:
- Safety Data Sheet:
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Questions about ROTI®Lumin, 2 x 25 ml, 10x conc., fluorescence-free
What is this article?
ROTI®Lumin, 2 x 25 ml, 10x conc., fluorescence-free is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Protein extraction, purification & markers, inside Protein biology & Western blot, in Microbiology lab supplies.
Extraction reagents break the cell and keep the protein soluble. Purification resins and columns, often an affinity tag such as His, pull one protein out. A marker is a set of known masses run in the next lane. The mass range of the marker has to cover the protein being sought.
Protein work separates a mixture, then asks which protein is which. Extraction and purification take the protein out of the cell. A marker shows the masses. A Western blot moves the separated proteins onto a membrane and probes them with an antibody. The membrane, nitrocellulose or PVDF, has to bind the protein and survive the washes. Antibodies and methods are in PubMed. Reference material is at ATCC.
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The category is Protein biology & Western blot. The sub-category is Protein extraction, purification & markers. The catalogue is Microbiology lab supplies.
Shelves beside this one are Blotting membranes & papers and Protein biology (other).
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The seven catalogues are Labware, Lab chemicals, Lab instruments, Lab consumables, Chromatography equipment, Lab safety equipment and Life Science & Microbiology.
